PUBLICATION
Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells
- Authors
- Senghaas, N., and Köster, R.W.
- ID
- ZDB-PUB-100223-4
- Date
- 2009
- Source
- CSH protocols 2009(6): pdb.prot5235 (Journal)
- Registered Authors
- Köster, Reinhard W., Senghaas, Niklas
- Keywords
- none
- MeSH Terms
-
- Zebrafish Proteins/isolation & purification
- Transfection/methods*
- Cryopreservation
- Cell Line
- Fibroblasts/cytology*
- Zebrafish/metabolism*
- Cell Survival
- Animals
- Tissue Fixation
- Cell Culture Techniques/methods*
- Immunohistochemistry
- PubMed
- 20147193 Full text @ CSH Protoc.
Citation
Senghaas, N., and Köster, R.W. (2009) Culturing and Transfecting Zebrafish PAC2 Fibroblast Cells. CSH protocols. 2009(6):pdb.prot5235.
Abstract
INTRODUCTION The use of zebrafish (Danio rerio) as a model organism has seen rapid growth in recent years. Numerous new techniques have emerged for zebrafish, and many protocols used in other model organisms have been adapted to fit the special needs of this species. Zebrafish cell lines, however, have not been studied to a great extent, and their applications remain limited. The PAC2 fibroblast line, isolated from 24-h post-fertilization zebrafish embryos, is one of the few available lines. Here, we provide a basic set of methods for maintenance and handling of PAC2 cells, as well as general procedures for transfection, immunocytochemistry, and protein extraction.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping