PUBLICATION
Identification of a heritable model of testicular germ cell tumor in the zebrafish
- Authors
- Neumann, J.C., Dovey, J.S., Chandler, G.L., Carbajal, L., and Amatruda, J.F.
- ID
- ZDB-PUB-100112-8
- Date
- 2009
- Source
- Zebrafish 6(4): 319-327 (Journal)
- Registered Authors
- Amatruda, James F., Carbajal, Liliana, Neumann, Joanie
- Keywords
- none
- MeSH Terms
-
- Animals
- Cell Differentiation
- Disease Models, Animal*
- Female
- Gene Expression Regulation, Neoplastic*
- Genetic Predisposition to Disease*
- Humans
- Male
- Mutation
- Neoplasms, Germ Cell and Embryonal/genetics*
- Neoplasms, Germ Cell and Embryonal/pathology
- Neoplasms, Germ Cell and Embryonal/radiotherapy
- Pedigree
- Zebrafish/genetics*
- PubMed
- 20047465 Full text @ Zebrafish
Citation
Neumann, J.C., Dovey, J.S., Chandler, G.L., Carbajal, L., and Amatruda, J.F. (2009) Identification of a heritable model of testicular germ cell tumor in the zebrafish. Zebrafish. 6(4):319-327.
Abstract
Germ cell tumors (GCTs) affect infants, children, and adults and are the most common cancer type in young men. Progress in understanding the molecular basis of GCTs has been hampered by a lack of suitable animal models. Here we report the identification of a zebrafish model of highly penetrant, heritable testicular GCT isolated as part of a forward genetic screen for cancer susceptibility genes. The mutant line develops spontaneous testicular tumors at a median age of 7 months, and pedigree analysis indicates dominant inheritance of the GCT susceptibility trait. The zebrafish model exhibits disruption of testicular tissue architecture and the accumulation of primitive, spermatogonial-like cells with loss of spermatocytic differentiation. Radiation treatment leads to apoptosis of the tumor cells and tumor regression. The GCT-susceptible line can serve as a model for understanding the mechanisms regulating germ cells in normal development and disease and as a platform investigating new therapeutic approaches for GCTs.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping