ZFIN ID: ZDB-PUB-091023-63
Osteoblast maturation occurs in overlapping proximal-distal compartments during fin regeneration in zebrafish
Brown, A.M., Fisher, S., and Iovine, M.K.
Date: 2009
Source: Developmental dynamics : an official publication of the American Association of Anatomists   238(11): 2922-2928 (Journal)
Registered Authors: Fisher, Shannon, Iovine, M. Kathryn
Keywords: bone growth, osteoblast maturation, fin regeneration, zebrafish, cx43
MeSH Terms:
  • Animals
  • Bone Regeneration*
  • Cell Differentiation/physiology*
  • Osteoblasts/cytology
  • Osteoblasts/physiology*
  • Osteogenesis/physiology*
  • Osteopontin/metabolism
  • Transcription Factors/metabolism
  • Zebrafish/physiology*
  • Zebrafish Proteins/metabolism
PubMed: 19842180 Full text @ Dev. Dyn.
During fin regeneration, osteoblasts must continually differentiate for outgrowth of the bony fin rays. Bone maturity increases in a distal-proximal manner, and osteoblast maturation can be detected similarly when following gene expression. We find that early markers for osteoblast differentiation are expressed in a discrete domain at the distal end of the fin, just proximal to the adjacent germinal compartment of dividing cells. Matrix genes, required at later stages developmentally, are expressed in a population of cells proximally to the early genes. A marker for mature osteoblasts is expressed in cells further proximal. These domains of gene expression are partially overlapping, perhaps revealing additional levels of osteoblast maturity. We suggest a model for growth where new cells are continually added to the distal-most osteoblast compartment, while osteoblasts in more proximal locations differentiate, thus translating developmental time to location on the proximal-distal axis.