PUBLICATION

Visualization of stochastic Ca2+ signals in the formed somites during the early segmentation period in intact, normally developing zebrafish embryos

Authors
Leung, C.F., Miller, A.L., Korzh, V., Chong, S.W., Sleptsova-Freidrich, I., and Webb, S.E.
ID
ZDB-PUB-090914-6
Date
2009
Source
Development, growth & differentiation   51(7): 617-637 (Journal)
Registered Authors
Chong, Shang Wei, Korzh, Vladimir, Leung, Christina F., Miller, Andrew L., Sleptsova-Freidrich, Inna, Webb, Sarah E.
Keywords
aequorin, Ca2+, convergence, somites, zebrafish
MeSH Terms
  • Animals
  • Calcium Channel Blockers/pharmacology
  • Calcium Signaling*/drug effects
  • Embryo, Nonmammalian/metabolism
  • Inositol 1,4,5-Trisphosphate Receptors/metabolism*
  • Somites/embryology
  • Somites/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
PubMed
19712266 Full text @ Dev. Growth Diff.
Abstract
Localized Ca(2+) signals were consistently visualized in the formed somites of intact zebrafish embryos during the early segmentation period. Unlike the regular process of somitogenesis, these signals were stochastic in nature with respect to time and location. They did, however, occur predominantly at the medial and lateral boundaries within the formed somites. Embryos were treated with modulators of [Ca(2+)](i) to explore the signal generation mechanism and possible developmental function of the stochastic transients. Blocking elements in the phosphoinositol pathway eliminated the stochastic signals but had no obvious effect, stochastic or otherwise, on the formed somites. Such treatments did, however, result in the subsequently formed somites being longer in the mediolateral dimension. Targeted uncaging of buffer (diazo-2) or Ca(2+) (NP-ethyleneglycoltetraacetic acid [EGTA]) in the presomitic mesoderm, resulted in a regular mediolateral lengthening and shortening, respectively, of subsequently formed somites. These data suggest a requirement for IP(3) receptor-mediated Ca(2+) release during convergence cell movements in the presomitic mesoderm, which appears to have a distinct function from that of the IP(3) receptor-mediated stochastic Ca(2+) signaling in the formed somites.
Genes / Markers
Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping