PUBLICATION

Olfactory neural circuitry for attraction to amino acids revealed by transposon-mediated gene trap approach in zebrafish

Authors
Koide, T., Miyasaka, N., Morimoto, K., Asakawa, K., Urasaki, A., Kawakami, K., and Yoshihara, Y.
ID
ZDB-PUB-090616-25
Date
2009
Source
Proceedings of the National Academy of Sciences of the United States of America   106(24): 9884-9889 (Journal)
Registered Authors
Kawakami, Koichi, Miyasaka, Nobuhiko, Morimoto, Kozo, Yoshihara, Yoshihiro
Keywords
feeding behavior, Gal4/UAS, neural transmission blockade, olfaction
MeSH Terms
  • Amino Acids/metabolism*
  • Animals
  • DNA Transposable Elements*
  • Immunohistochemistry
  • Olfactory Pathways*
  • Zebrafish/genetics
  • Zebrafish/physiology*
PubMed
19497864 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
In fish, amino acids are food-related important olfactory cues to elicit an attractive response. However, the neural circuit underlying this olfactory behavior is not fully elucidated. In the present study, we applied the Tol2 transposon-mediated gene trap method to dissect the zebrafish olfactory system genetically. Four zebrafish lines (SAGFF27A, SAGFF91B, SAGFF179A, and SAGFF228C) were established in which the modified transcription activator Gal4FF was expressed in distinct subsets of olfactory sensory neurons (OSNs). The OSNs in individual lines projected axons to partially overlapping but mostly different glomeruli in the olfactory bulb (OB). In SAGFF27A, Gal4FF was expressed predominantly in microvillous OSNs innervating the lateral glomerular cluster that corresponded to the amino acid-responsive region in the OB. To clarify the olfactory neural pathway mediating the feeding behavior, we genetically expressed tetanus neurotoxin in the Gal4FF lines to block synaptic transmission in distinct populations of glomeruli and examined their behavioral response to amino acids. The attractive response to amino acids was abolished only in SAGFF27A fish carrying the tetanus neurotoxin transgene. These findings clearly demonstrate the functional significance of the microvillous OSNs innervating the lateral glomerular cluster in the amino acid-mediated feeding behavior of zebrafish. Thus, the integrated approach combining genetic, neuroanatomical, and behavioral methods enables us to elucidate the neural circuit mechanism underlying various olfactory behaviors in adult zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping