PUBLICATION

Genetic Evidence for a Noncanonical Function of Seryl-tRNA Synthetase in Vascular Development

Authors
Herzog, W., Müller, K., Huisken, J., and Stainier, D.Y.
ID
ZDB-PUB-090511-23
Date
2009
Source
Circulation research   104(11): 1260-1266 (Journal)
Registered Authors
Herzog, Wiebke, Stainier, Didier
Keywords
aminoacyl-tRNA synthetases, seryl-tRNA synthetase, Sars, SerRS, zebrafish, angiogenesis, vascular dilatation
MeSH Terms
  • Angiogenesis Inhibitors/pharmacology
  • Animals
  • Aorta, Thoracic/cytology
  • Aorta, Thoracic/embryology
  • Aorta, Thoracic/physiology
  • Brain/physiology
  • Cell Division
  • Cerebrovascular Circulation/physiology*
  • Chromosome Mapping
  • Cycloheximide/pharmacology
  • Embryo, Nonmammalian/physiology
  • Endothelium, Vascular/cytology
  • Endothelium, Vascular/physiology
  • Heart/embryology*
  • Humans
  • Indoles/pharmacology
  • Myocardium/cytology
  • Pyrroles/pharmacology
  • RNA, Small Interfering/genetics
  • Serine-tRNA Ligase/genetics*
  • Transfection
  • Umbilical Veins/cytology
  • Umbilical Veins/physiology
  • Zebrafish
PubMed
19423847 Full text @ Circ. Res.
Abstract
In a recent genetic screen, we identified mutations in genes important for vascular development and maintenance in zebrafish (Jin et al. Dev Biol. 2007;307:29-42). 32 Mutations at the adrasteia (adr) locus cause a pronounced dilatation of the aortic arch vessels as well as aberrant patterning of the hindbrain capillaries and, to a lesser extent, intersomitic vessels. This dilatation of the aortic arch vessels does not appear to be caused by increased cell proliferation but is dependent on vascular endothelial growth factor (Vegf) signaling. By positional cloning, we isolated seryl-tRNA synthetase (sars) as the gene affected by the adr mutations. Small interfering RNA knockdown experiments in human umbilical vein endothelial cell cultures indicate that SARS also regulates endothelial sprouting. These analyses of zebrafish and human endothelial cells reveal a new noncanonical function of Sars in endothelial development.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes