PUBLICATION
A primer for morpholino use in zebrafish
- Authors
- Bill, B.R., Petzold, A.M., Clark, K.J., Schimmenti, L.A., and Ekker, S.C.
- ID
- ZDB-PUB-090422-16
- Date
- 2009
- Source
- Zebrafish 6(1): 69-77 (Review)
- Registered Authors
- Bill, Brent, Clark, Karl, Ekker, Stephen C., Petzold, Andrew
- Keywords
- none
- MeSH Terms
-
- Animals
- Gene Knockdown Techniques*
- Oligonucleotides/genetics*
- Zebrafish/genetics*
- PubMed
- 19374550 Full text @ Zebrafish
Citation
Bill, B.R., Petzold, A.M., Clark, K.J., Schimmenti, L.A., and Ekker, S.C. (2009) A primer for morpholino use in zebrafish. Zebrafish. 6(1):69-77.
Abstract
Morpholino oligonucleotides are the most common anti-sense "knockdown" technique used in zebrafish (Danio rerio). This review discusses common practices for the design, preparation, and deployment of morpholinos in this vertebrate model system. Off-targeting effects of morpholinos are discussed as well as method to minimize this potentially confounding variable via co-injection of a tP53-targeting morpholino. Finally, new uses of morpholinos are summarized and contextualized with respect to the complementary, DNA-based knockout technologies recently developed for zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping