PUBLICATION
The essential role of endogenous ghrelin in growth hormone expression during zebrafish adenohypophysis development
- Authors
- Li, X., He, J., Hu, W., and Yin, Z.
- ID
- ZDB-PUB-090310-14
- Date
- 2009
- Source
- Endocrinology 150(6): 2767-2774 (Journal)
- Registered Authors
- He, Jiangyan, Hu, Wei, Li, Xi, Yin, Zhan
- Keywords
- ghrelin, embryonic development, growth hormone expression, acylated-ghrelin, obestatin
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Embryonic Development/genetics
- Embryonic Development/physiology*
- Ghrelin/genetics
- Ghrelin/metabolism
- Ghrelin/physiology*
- Growth Hormone/metabolism*
- Molecular Sequence Data
- Oligonucleotides/pharmacology
- Pituitary Gland, Anterior/embryology*
- Pituitary Gland, Anterior/metabolism*
- Protein Biosynthesis/drug effects
- RNA, Messenger/metabolism
- Zebrafish/physiology*
- PubMed
- 19264876 Full text @ Endocrinology
Citation
Li, X., He, J., Hu, W., and Yin, Z. (2009) The essential role of endogenous ghrelin in growth hormone expression during zebrafish adenohypophysis development. Endocrinology. 150(6):2767-2774.
Abstract
Ghrelin, a multi-functional hormone, including potent growth hormone stimulation activity, has been suggested to be important during embryonic development. Expression of ghrelin has been confirmed in the zebrafish pancreas during embryonic stages. Interfering with ghrelin function using two specific antisense morpholino oligonucleotides causes defects during zebrafish embryonic development. In ghrelin morphants, the expression of growth hormone was abolished in zebrafish somatotropes, while the expression patterns of the other key molecules involved in hypothalamic-pituitary development and distinct pituitary hormones genes remain largely intact at the appropriate time during zebrafish adenohypophysis development. Effective rescue of the ghrelin morphants with exogenous ghrelin mRNA showed that the correct gene had been targeted. Moreover, by analyzing the efficiencies of the ghrelin morphants rescue experiments with various forms of exogenous mutant ghrelin mRNAs, we also demonstrated the essentiality of the form acyl-ghrelin on growth hormone stimulation during zebrafish adenohypophysis development. Our in vivo experiments, for the first time, also provided evidence of the existence of functional obestatin in the C-terminal part of zebrafish proghrelin peptides. Our research here has demonstrated that zebrafish is a unique model for functional studies of endogenous ghrelin, especially during embryonic development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping