PUBLICATION

Positive Darwinian selection and the birth of an olfactory receptor clade in teleosts

Authors
Hussain, A., Saraiva, L.R., and Korsching, S.I.
ID
ZDB-PUB-090302-20
Date
2009
Source
Proceedings of the National Academy of Sciences of the United States of America   106(11): 4313-4318 (Journal)
Registered Authors
Korsching, Sigrun, Saraiva, Luis
Keywords
Danio rerio, evolution, in situ hybridization, shark, trace amine
MeSH Terms
  • Animals
  • Evolution, Molecular*
  • Fishes
  • Olfactory Receptor Neurons
  • Phylogeny
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, Odorant/genetics*
  • Selection, Genetic*
PubMed
19237578 Full text @ Proc. Natl. Acad. Sci. USA
Abstract
Trace amine-associated receptors (TAARs) in mammals recently have been shown to function as olfactory receptors. We have delineated the taar gene family in jawless, cartilaginous, and bony fish (zero, 2, and >100 genes, respectively). We conclude that taar genes are evolutionary much younger than the related OR and ORA/V1R olfactory receptor families, which are present already in lamprey, a jawless vertebrate. The 2 cartilaginous fish genes appear to be ancestral for 2 taar classes, each with mammalian and bony fish (teleost) representatives. Unexpectedly, a whole new clade, class III, of taar genes originated even later, within the teleost lineage. Taar genes from all 3 classes are expressed in subsets of zebrafish olfactory receptor neurons, supporting their function as olfactory receptors. The highly conserved TAAR1 (shark, mammalian, and teleost orthologs) is not expressed in the olfactory epithelium and may constitute the sole remnant of a primordial, nonolfactory function of this family. Class III comprises three-fourths of all teleost taar genes and is characterized by the complete loss of the aminergic ligand-binding motif, stringently conserved in the other 2 classes. Two independent intron gains in class III taar genes represent extraordinary evolutionary dynamics, considering the virtual absence of intron gains during vertebrate evolution. The d(N)/d(S) analysis suggests both minimal global negative selection and an unparalleled degree of local positive selection as another hallmark of class III genes. The accelerated evolution of class III teleost taar genes conceivably might mark the birth of another olfactory receptor gene family.
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