ZFIN ID: ZDB-PUB-090204-2
gfap and nestin reporter lines reveal characteristics of neural progenitors in the adult zebrafish brain
Lam, C.S., Maerz, M., and Strähle, U.
Date: 2009
Source: Developmental dynamics : an official publication of the American Association of Anatomists   238(2): 475-486 (Journal)
Registered Authors: Maerz, Martin, Strähle, Uwe
Keywords: adult neurogenesis, ventricular zone, telencephalon, transgenic line
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain/cytology
  • Brain/metabolism*
  • Genes, Reporter
  • Glial Fibrillary Acidic Protein/genetics
  • Glial Fibrillary Acidic Protein/metabolism*
  • Intermediate Filament Proteins/genetics
  • Intermediate Filament Proteins/metabolism*
  • Nerve Tissue Proteins/genetics
  • Nerve Tissue Proteins/metabolism*
  • Nestin
  • Neurons/cytology*
  • Neurons/metabolism
  • Prosencephalon/cytology
  • Prosencephalon/metabolism
  • SOXB1 Transcription Factors/genetics
  • Stem Cells/cytology*
  • Stem Cells/metabolism
  • Zebrafish/metabolism*
PubMed: 19161226 Full text @ Dev. Dyn.
Adult neurogenesis arises from niches that harbor neural stem cells (NSC). Although holding great promise for regenerative medicine, the identity of NSC remains elusive. In mammals, a key attribute of NSC is the expression of the filamentous proteins glial fibrillary acidic protein (GFAP) and NESTIN. To assess whether these two markers are relevant in the fish model, two transgenic zebrafish lines for gfap and nestin were generated. Analysis of adult brains showed that the fusion GFAP-green fluorescent protein closely mimics endogenous GFAP, while the nestin transgene recapitulates nestin at the ventricular zones. Cells expressing the two reporters display radial glial morphology, colocalize with the NSC marker Sox2, undergo proliferation, and are capable of self-renewal within the matrix of distinct thickness in the telencephalon. Together, these two transgenic lines reveal a conserved feature of putative NSC in the adult zebrafish brain and provide a means for the identification and manipulation of these cells in vivo.