PUBLICATION

Identification and characterization of two zebrafish nectin-1 genes that are differentially expressed in the developing eye and brain

Authors
Helvik, J.V., Rødahl, E., Drivenes, O., and Haarr, L.
ID
ZDB-PUB-090106-3
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(1): 43-55 (Journal)
Registered Authors
Drivenes, Oyvind
Keywords
cell adhesion, retina, CNS, neurodevelopment, poliovirus receptor, teleost
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Brain/anatomy & histology
  • Brain/embryology*
  • Brain/physiology
  • Cell Adhesion Molecules/classification
  • Cell Adhesion Molecules/genetics
  • Cell Adhesion Molecules/metabolism*
  • Eye/anatomy & histology
  • Eye/enzymology*
  • Eye/metabolism
  • Gene Expression Regulation, Developmental*
  • Humans
  • In Situ Hybridization
  • Molecular Sequence Data
  • Phylogeny
  • Protein Isoforms/classification
  • Protein Isoforms/genetics
  • Protein Isoforms/metabolism*
  • Sequence Alignment
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Zebrafish Proteins/classification
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
19097185 Full text @ Dev. Dyn.
Abstract
Nectins are cell adhesion molecules of the immunoglobulin type that play important roles in the development of the nervous system. We have characterized two paralogous zebrafish nectin-1 genes, nectin-1a and nectin-1b, that differ in expression. Nectin-1a expression is first found in the anterior neural keel and later in the optic cup. In the retina, nectin-1a appears in the outer part and extends inwards, while nectin-1b starts in the inner part and spreads outwards. Only nectin-1a was detected in the cornea, the lens, and in the region of photoreceptor cell differentiation in the retina. Both genes were expressed in ganglion cells and inner nuclear neurons. In the brain, nectin-1a was restricted to the epiphysis and a cluster of cells in the posterior hindbrain, whereas nectin-1b was found in several brain areas. Zebrafish may, therefore, be a useful model for identifying different functions of nectin-1 in the developing eye and nervous system.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping