PUBLICATION

The extracellular matrix protein TGFBI promotes myofibril bundling and muscle fibre growth in the zebrafish embryo

Authors
Kim, H.R., and Ingham, P.W.
ID
ZDB-PUB-090106-1
Date
2009
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   238(1): 56-65 (Journal)
Registered Authors
Ingham, Philip, Kim, Rosemary
Keywords
Tgfbi, extracellular matrix, skeletal muscle, myofibril, zebrafish, embryo
MeSH Terms
  • Zebrafish*/anatomy & histology
  • Zebrafish*/embryology
  • Extracellular Matrix Proteins/genetics
  • Extracellular Matrix Proteins/metabolism*
  • Muscle Fibers, Skeletal/cytology
  • Muscle Fibers, Skeletal/physiology*
  • Myofibrils/physiology*
  • Myofibrils/ultrastructure
  • Mice
  • Oligonucleotides, Antisense/genetics
  • Oligonucleotides, Antisense/metabolism
  • Humans
  • Transforming Growth Factor beta/genetics
  • Transforming Growth Factor beta/metabolism*
  • Recombinant Fusion Proteins/genetics
  • Recombinant Fusion Proteins/metabolism
  • Extracellular Matrix/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Animals
(all 20)
PubMed
19097068 Full text @ Dev. Dyn.
Abstract
The extracellular matrix protein Tgfbi has been shown to localise to myotendinous junctions in mouse and postulated to interact with the transmembrane protein Integrin alpha7beta1, which, in parallel with the Dystrophin-associated protein complex, is critical for linkage between the extracellular matrix and the cytoskeleton of muscle fibres. Here we use a GFP-tagged form of Tgfbi to analyse its distribution in the developing skeletal muscle of the zebrafish embryos and antisense morpholino oligonucleotides to investigate the function of the endogenous protein. We find that although tagged Tgfbi accumulates at the myosepta, the attachment of muscle fibres to the myosepta is established and maintained normally in morphant embryos; however, the fibres show a marked reduction in their growth and a disruption of their myofibril bundles.
Genes / Markers
Figures
Figure Gallery (11 images) / 2
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Expression
Phenotype
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
i104TgTransgenic Insertion
    zf13TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      tgfbiMO1-tgfbiMRPHLNO
      tgfbiMO2-tgfbiMRPHLNO
      1 - 2 of 2
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      Fish
      Antibodies
      Orthology
      No data available
      Engineered Foreign Genes
      Marker Marker Type Name
      EGFPEFGEGFP
      GFPEFGGFP
      1 - 2 of 2
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      Mapping
      No data available