|ZFIN ID: ZDB-PUB-081218-17|
Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo
Tseng, D.Y., Chou, M.Y., Tseng, Y.C., Hsiao, C.D., Huang, C.J., Kaneko, T., and Hwang, P.P.
|Source:||American journal of physiology. Regulatory, integrative and comparative physiology 296(3): R549-R557 (Journal)|
|Registered Authors:||Chou, Ming-Yi, Hsiao, Chung-Der, Huang, Chang-Jen, Hwang, Pung Pung, Tseng, Deng-Yu, Tseng, Yung-Che|
|Keywords:||Stanniocalcin, Ca2+ uptake, ECaC, Ca2+ influx, zebrafish|
|PubMed:||19073903 Full text @ Am. J. Physiol. Regul. Integr. Comp. Physiol.|
Tseng, D.Y., Chou, M.Y., Tseng, Y.C., Hsiao, C.D., Huang, C.J., Kaneko, T., and Hwang, P.P. (2009) Effects of Stanniocalcin 1 on calcium uptake in zebrafish (Danio rerio) embryo. American journal of physiology. Regulatory, integrative and comparative physiology. 296(3):R549-R557.
ABSTRACTStanniocalcin (STC, formerly called hypocalcin or teleocalcin) is a 50-kDa disulfide-linked homodimeric glycoprotein that was originally identified in fish and secreted from the corpuscles of Stannius (CS). One of the main functions of STC-1 is Ca(2+) uptake inhibition; however the mechanisms remain unknown. In the present study, we provide molecular evidence to elucidate how zebrafish STC-1 regulates Ca(2+) uptake in zebrafish embryos. zstc-1 was expressed in a wide variety of tissues including the kidney, brain, gill, muscle, and skin. Incubating zebrafish embryos in low-Ca(2+) (0.02 mM) freshwater stimulated whole-body Ca(2+) influx and zebrafish epithelial Ca(2+) channel (zECaC) mRNA expression while down-regulated zstc-1 expression. A morpholino microinjection approach was used to knockdown the zSTC-1 protein, and the results showed that the Ca(2+) content, Ca(2+) influx, and zECaC mRNA expression all increased in morphants. These data suggest that zSTC-1 negatively regulates epithelial Ca(2+) channel (ECaC) gene expression to reduce Ca(2+) uptake in zebrafish embryos. Key words: Stanniocalcin, zebrafish, Ca2+ influx, ECaC.