PUBLICATION
Novel caspase-suicide proteins for tamoxifen-inducible apoptosis
- Authors
- Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R.
- ID
- ZDB-PUB-080922-22
- Date
- 2008
- Source
- Genesis (New York, N.Y. : 2000) 46(10): 530-536 (Journal)
- Registered Authors
- Köster, Reinhard W.
- Keywords
- caspase, apoptosis, tamoxifen, cell ablation, estrogen receptor
- MeSH Terms
-
- Animals
- Antineoplastic Agents, Hormonal/pharmacology*
- Apoptosis/drug effects*
- Apoptosis/genetics
- Binding Sites/drug effects
- Binding Sites/genetics
- Caspases/genetics
- Caspases/physiology*
- Cell Line
- Cell Line, Transformed
- Genes, Transgenic, Suicide*/drug effects
- HeLa Cells
- Humans
- Mice
- Selective Estrogen Receptor Modulators/pharmacology
- Tamoxifen/pharmacology*
- Zebrafish
- PubMed
- 18802959 Full text @ Genesis
Citation
Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R. (2008) Novel caspase-suicide proteins for tamoxifen-inducible apoptosis. Genesis (New York, N.Y. : 2000). 46(10):530-536.
Abstract
Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping