PUBLICATION
Novel caspase-suicide proteins for tamoxifen-inducible apoptosis
- Authors
- Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R.
- ID
- ZDB-PUB-080922-22
- Date
- 2008
- Source
- Genesis (New York, N.Y. : 2000) 46(10): 530-536 (Journal)
- Registered Authors
- Köster, Reinhard W.
- Keywords
- caspase, apoptosis, tamoxifen, cell ablation, estrogen receptor
- MeSH Terms
-
- Zebrafish
- Mice
- Cell Line
- Caspases/genetics
- Caspases/physiology*
- Binding Sites/drug effects
- Binding Sites/genetics
- HeLa Cells
- Selective Estrogen Receptor Modulators/pharmacology
- Genes, Transgenic, Suicide*/drug effects
- Apoptosis/drug effects*
- Apoptosis/genetics
- Cell Line, Transformed
- Humans
- Antineoplastic Agents, Hormonal/pharmacology*
- Tamoxifen/pharmacology*
- Animals
- PubMed
- 18802959 Full text @ Genesis
Citation
Chu, Y., Senghaas, N., Köster, R.W., Wurst, W., and Kühn, R. (2008) Novel caspase-suicide proteins for tamoxifen-inducible apoptosis. Genesis (New York, N.Y. : 2000). 46(10):530-536.
Abstract
Taking advantage of a mutant estrogen receptor ligand binding domain (ER(T2)), we developed novel Caspase fusion proteins for inducible apoptosis. We show that Caspase-ER(T2) fusion proteins become specifically activated by the synthetic ligand 4-OH- tamoxifen and rapidly induce apoptotic cell death in human, murine, and zebrafish cells. This novel tool for targeted cell ablation greatly facilitates the generation of disease models as well as developmental and regeneration studies in model organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping