|ZFIN ID: ZDB-PUB-080801-6|
PCB126 Exposure Disrupts Zebrafish Ventricular and Branchial but Not Early Neural Crest Development
Grimes, A.C., Erwin, K.N., Stadt, H.A., Hunter, G.L., Gefroh, H.A., Tsai, H.J., and Kirby, M.L.
|Source:||Toxicological sciences : an official journal of the Society of Toxicology 106(1): 193-205 (Journal)|
|Registered Authors:||Grimes, Adrian, Kirby, Margaret L., Tsai, Huai-Jen|
|Keywords:||zebrafish, ventricular development, valvulogenesis, cardiotoxicity, PCB126, aryl hydrocarbon receptor, proliferation, branchial cartilages|
|PubMed:||18660518 Full text @ Toxicol. Sci.|
Grimes, A.C., Erwin, K.N., Stadt, H.A., Hunter, G.L., Gefroh, H.A., Tsai, H.J., and Kirby, M.L. (2008) PCB126 Exposure Disrupts Zebrafish Ventricular and Branchial but Not Early Neural Crest Development. Toxicological sciences : an official journal of the Society of Toxicology. 106(1):193-205.
ABSTRACTWe have used zebrafish and 3,3',4,4',5-pentachlorobiphenyl (PCB126) to investigate the developmental toxicity of PCBs that exert their effects through the aryl hydrocarbon receptor (AHR). We found that cardiac and neural crest-derived jaw and branchial cartilages are specifically targeted early in development. The suite of malformations include a severely dysmorphic heart with a reduced bulbus arteriosus, abnormal atrioventricular and outflow valve formation, ultimately leading to circulatory failure. Early neural crest migration and patterning of the jaw and branchial cartilages was normal. However, the jaw and branchial cartilages failed to grow to normal size. In the heart, the ventricular myocardium showed a reduction in cell number and size. The heart and jaw/branchial phenotype could be rescued by pifithrin-alpha, a blocker of p53. However, the function of pifithrin-alpha in this model may act as a competitive inhibitor of PCB at the AHR and is independent of p53. Morpholinos against p53 did not rescue the phenotype, nor were zebrafish with a mutant p53-null allele resistant to PCB126 toxicity. Morpholino knock-down of cardiac troponin T, which blocks the onset of cardiac function, prevented the PCB126-induced cardiac dysmorphogenesis but not the jaw/branchial phenotype. The cardiovascular characteristics appear to be similar to hypoplastic left heart syndrome (HLHS) and introduce the potential of zebrafish as a model to study this environmentally induced cardiovascular malformation. HLHS is a severe congenital cardiovascular malformation that has previously been linked to industrial releases of dioxins and polychlorinated biphenyls (PCBs).