PUBLICATION
meis1 regulates the development of endothelial cells in zebrafish
- Authors
- Minehata, K.I., Kawahara, A., and Suzuki, T.
- ID
- ZDB-PUB-080801-2
- Date
- 2008
- Source
- Biochemical and Biophysical Research Communications 374(4): 647-652 (Journal)
- Registered Authors
- Kawahara, Atsuo
- Keywords
- meis1, flk1, vegf, Vascular development, Dorsal aorta, Zebrafish
- MeSH Terms
-
- Animals
- RNA, Antisense/genetics
- Transcription Factors/genetics
- Transcription Factors/physiology*
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/immunology
- Endothelium, Vascular/cytology
- Endothelium, Vascular/embryology*
- Endothelium, Vascular/metabolism
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology*
- Vascular Endothelial Growth Factor Receptor-2/genetics
- Vascular Endothelial Growth Factor Receptor-2/metabolism
- Embryonic Development
- Cell Differentiation*
- Phenotype
- Blood Vessels/cytology
- Blood Vessels/embryology*
- Blood Vessels/metabolism
- PubMed
- 18656453 Full text @ Biochem. Biophys. Res. Commun.
Citation
Minehata, K.I., Kawahara, A., and Suzuki, T. (2008) meis1 regulates the development of endothelial cells in zebrafish. Biochemical and Biophysical Research Communications. 374(4):647-652.
Abstract
The differentiation of endothelial cells is tightly connected with the formation of blood vessels during vertebrate development. The signaling pathways mediated by vascular endothelial growth factor (vegf) are required for these processes. Here we show that a proto-oncogene, meis1, plays important roles in the vascular development in zebrafish. Knockdown of meis1 by anti-sense meis1 morpholino (meis1 MO) led to the impairment of intersegmental vessel (ISV) formation. In meis1 morphants, the expression of an artery marker was reduced in dorsal aorta (DA), and the expression of vein markers was expanded in DA and posterior cardinal vein (PCV), suggesting the defects on artery development. Furthermore, the expression of vegf receptor, flk1, was significantly decreased in these embryos. Interestingly, flk1 MO-injected embryos exhibited similar defects as meis1 morphants. Thus, these results implicate that meis1 is a novel regulator involved in endothelial cell development, presumably affecting the vegf signaling pathway.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping