PUBLICATION
Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos
- Authors
- Pei, D.S., Sun, Y.H., and Zhu, Z.Y.
- ID
- ZDB-PUB-080728-17
- Date
- 2009
- Source
- Molecular biology reports 36(6): 1375-1380 (Journal)
- Registered Authors
- Pei, Desheng, Sun, Yonghua, Zhu, Zuoyan
- Keywords
- Nuclear reprogramming, Nuclear transfer, Rare minnow (Gobiocypris rarus), Zebrafish (Danio rerio)
- MeSH Terms
-
- Ovum
- Polymerase Chain Reaction
- Animals
- Phosphorylation
- RNA, Messenger/analysis
- Embryo, Nonmammalian
- Zebrafish/genetics*
- Genes, Developmental*
- Computational Biology
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Gene Expression Regulation, Developmental*
- Female
- Molecular Sequence Data
- Clone Cells
- Base Sequence
- Gene Components
- Phosphoproteins/genetics*
- Phosphoproteins/metabolism
- PubMed
- 18654838 Full text @ Mol. Biol. Rep.
Citation
Pei, D.S., Sun, Y.H., and Zhu, Z.Y. (2009) Identification of a novel gene K23 over-expressed in fish cross-subfamily cloned embryos. Molecular biology reports. 36(6):1375-1380.
Abstract
A novel gene-K23, differentially expressed in cross-subfamily cloned embryos, was isolated by RACE-PCR technique. It had 2580 base pairs (bp) in length, with a 1,425 bp open reading frame (ORF) encoding a putative protein of 474 amino acids (aa). Bioinformatic analysis indicated that K23 had 22 phosphorylation sites, but it had no signal peptides. Developmental expression analysis in zebrafish showed that K23 transcripts were maternally expressed in ovum and the amount of K23 transcripts increased gradually from zygote to pharyngula period. Subcellular localization analysis revealed that K23 protein was homogeneously distributed both in nuclei and cytoplasm. Taken together, our findings indicate that K23 gene is a novel gene differentially expressed in fish cross-subfamily cloned embryos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping