PUBLICATION

Reiterative roles for FGF signaling in the establishment of size and proportion of the zebrafish heart

Authors
Marques, S.R., Lee, Y., Poss, K.D., and Yelon, D.
ID
ZDB-PUB-080722-27
Date
2008
Source
Developmental Biology   321(2): 397-406 (Journal)
Registered Authors
Lee, Yoonsung, Poss, Kenneth D., Yelon, Deborah
Keywords
zebrafish, organogenesis, heart development, chamber formation, ventricle, atrium, FGF, Fgf8, acerebellar
MeSH Terms
  • Fibroblast Growth Factors/metabolism*
  • Heart/embryology*
  • Heart/physiology
  • Signal Transduction/physiology*
  • Fluorescent Antibody Technique
  • Animals
  • Zebrafish/embryology*
  • Pyrroles
  • Myocytes, Cardiac/physiology
  • Cell Differentiation/physiology
  • In Situ Hybridization
  • Organ Size
  • Transcription Factors/metabolism
(all 13)
PubMed
18639539 Full text @ Dev. Biol.
Abstract
Development of a functional organ requires the establishment of its proper size as well as the establishment of the relative proportions of its individual components. In the zebrafish heart, organ size and proportion depend heavily on the number of cells in each of its two major chambers, the ventricle and the atrium. Heart size and chamber proportionality are both affected in zebrafish fgf8 mutants. To determine when and how FGF signaling influences these characteristics, we examined the effect of temporally controlled pathway inhibition. During cardiac specification, reduction of FGF signaling inhibits formation of both ventricular and atrial cardiomyocytes, with a stronger impact on ventricular cells. After cardiomyocyte differentiation begins, reduction of FGF signaling can still result in a deficiency of ventricular cardiomyocytes. Consistent with two temporally distinct roles for FGF, we find that increased FGF signaling induces a cardiomyocyte surplus only before cardiac differentiation begins. Thus, FGF signaling first regulates heart size and chamber proportionality during cardiac specification and later refines ventricular proportion by regulating cell number after the onset of differentiation. Together, our data demonstrate that a single signaling pathway can act reiteratively to coordinate organ size and proportion.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Phenotype
Fish Conditions Stage Phenotype Figure
fgf8ati282a/ti282astandard conditions20-25 somites
fgf8ati282a/ti282a; f2Tgstandard conditionsLong-pec
fgf8ati282a/ti282a; f2Tgstandard conditionsLong-pec
fgf8ati282a/ti282a; f2Tgstandard conditionsLong-pec
fgf8ati282a/ti282a; f2Tgstandard conditionsLong-pec
fgf8ati282a/ti282a; f2Tgstandard conditionsLong-pec
1 - 6 of 6
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Mutations / Transgenics
Allele Construct Type Affected Genomic Region
f2TgTransgenic Insertion
    pd3TgTransgenic Insertion
      ti282a
        		Point Mutation
        1 - 3 of 3
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        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        No data available
        Fish
        Fish
        f2Tg
        f2Tg; pd3Tg
        fgf8ati282a/ti282a
        fgf8ati282a/ti282a; f2Tg
        pd3Tg
        WT
        1 - 6 of 6
        Show
        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        DsRedEFGDsRed
        DsRed2EFGDsRed2
        1 - 2 of 2
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        Mapping
        No data available
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        Citation
        Marques, S.R., Lee, Y., Poss, K.D., and Yelon, D. (2008) Reiterative roles for FGF signaling in the establishment of size and proportion of the zebrafish heart. Developmental Biology. 321(2):397-406.