PUBLICATION

Embryonic requirements for ErbB signaling in neural crest development and adult pigment pattern formation

Authors

Budi, E.H., Patterson, L.B., and Parichy, D.M.

ID

ZDB-PUB-080602-12

Date

2008

Source

Development (Cambridge, England) 135(15): 2603-2614 (Journal)

Registered Authors

Budi, Erine, Parichy, David M.

Keywords

Erbb, erbb3, HER3, Melanophore, Metamorphosis, Stem cell, Zebrafish

MeSH Terms

Embryo, Nonmammalian/embryology*
Embryo, Nonmammalian/metabolism*
Gene Expression Regulation, Developmental
Skin Pigmentation/drug effects
Larva/cytology
Larva/genetics
Larva/growth & development
Larva/metabolism
Melanophores/cytology
Melanophores/drug effects
Melanophores/metabolism
Aging/physiology*
Neural Crest/cytology
Neural Crest/embryology
Neural Crest/growth & development
Neural Crest/metabolism*
Zebrafish/embryology
Zebrafish/growth & development
Zebrafish/metabolism*
Mutation/genetics
Base Sequence
Cell Movement
Cell Lineage
Alleles
Animals
Protein Kinase Inhibitors/pharmacology
Signal Transduction*
Receptor, ErbB-3/antagonists & inhibitors
Receptor, ErbB-3/genetics
Receptor, ErbB-3/metabolism*

(all 30)

PubMed

18508863 Full text @ Development

Abstract

Vertebrate pigment cells are derived from neural crest cells and are a useful system for studying neural crest-derived traits during post-embryonic development. In zebrafish, neural crest-derived melanophores differentiate during embryogenesis to produce stripes in the early larva. Dramatic changes to the pigment pattern occur subsequently during the larva-to-adult transformation, or metamorphosis. At this time, embryonic melanophores are replaced by newly differentiating metamorphic melanophores that form the adult stripes. Mutants with normal embryonic/early larval pigment patterns but defective adult patterns identify factors required uniquely to establish, maintain or recruit the latent precursors to metamorphic melanophores. We show that one such mutant, picasso, lacks most metamorphic melanophores and results from mutations in the ErbB gene erbb3b, which encodes an EGFR-like receptor tyrosine kinase. To identify critical periods for ErbB activities, we treated fish with pharmacological ErbB inhibitors and also knocked down erbb3b by morpholino injection. These analyses reveal an embryonic critical period for ErbB signaling in promoting later pigment pattern metamorphosis, despite the normal patterning of embryonic/early larval melanophores. We further demonstrate a peak requirement during neural crest migration that correlates with early defects in neural crest pathfinding and peripheral ganglion formation. Finally, we show that erbb3b activities are both autonomous and non-autonomous to the metamorphic melanophore lineage. These data identify a very early, embryonic, requirement for erbb3b in the development of much later metamorphic melanophores, and suggest complex modes by which ErbB signals promote adult pigment pattern development.

Genes / Markers

Figures

Show all Figures

Expression

Phenotype

Fish	Conditions	Stage	Phenotype	Figure
WT	chemical treatment: tyrphostin AG 1478	Long-pec	developmental pigmentation arrested, abnormal	Fig. 9
WT + MO1-erbb3b	standard conditions	Adult	developmental pigmentation arrested, abnormal	Fig. 7
csf1ra^j4e1/j4e1	control	Days 14-20	melanocyte amount, normal	Fig. 12
csf1ra^j4e1/j4e1	chemical treatment: tyrphostin AG 1478	Days 14-20	melanocyte decreased amount, abnormal	Fig. 12
erbb3b^utr2e1/+	standard conditions	Days 14-20 to Days 30-44	developmental pigmentation process quality, normal	Fig. 1
erbb3b^utr2e1/+	standard conditions	Days 14-20 to Days 30-44	trunk melanophore stripe has normal numbers of parts of type melanocyte, normal	Fig. 1
erbb3b^{utr2e1/utr2e1}	standard conditions	Days 14-20 to Days 21-29	post-vent region melanocyte poorly differentiated, abnormal	Fig. 2
erbb3b^{utr2e1/utr2e1}	standard conditions	Days 14-20 to Days 21-29	post-vent region melanophore stripe decreased pigmentation, abnormal	Fig. 2
erbb3b^{utr2e1/utr2e1}	standard conditions	Days 14-20 to Days 21-29	post-vent region melanophore stripe has fewer parts of type melanocyte, abnormal	Fig. 2
erbb3b^{utr2e1/utr2e1}	standard conditions	Days 14-20 to Days 30-44	developmental pigmentation arrested, abnormal	Fig. 1

1 - 10 of 33

Show

Mutations / Transgenics

Allele	Type	Affected Genomic Region
b5	Small Deletion	kita
j4e1	Point Mutation	csf1ra
utr2e1	Point Mutation	erbb3b
wpr2e2	Point Mutation	erbb3b

Human Disease / Model

Sequence Targeting Reagents

Target	Reagent	Reagent Type
erbb3b	MO1-erbb3b	MRPHLNO

Fish

Fish
csf1ra^j4e1/j4e1
erbb3b^utr2e1/+
erbb3b^{utr2e1/utr2e1}
erbb3b^wpr2e2/+
erbb3b^{wpr2e2/wpr2e2}
kita^b5/b5
WT
WT + MO1-erbb3b

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Budi et al., 2008 ZDB-PUB-080602-12 PMID:18508863

Embryonic requirements for ErbB signaling in neural crest development and adult pigment pattern formation

Budi et al., 2008

ZDB-PUB-080602-12
PMID:18508863