PUBLICATION

An Acyltransferase Controls the Generation of Hematopoietic and Endothelial Lineages in Zebrafish

Authors
Xiong, J.W., Yu, Q., Zhang, J., and Mably, J.D.
ID
ZDB-PUB-080414-6
Date
2008
Source
Circulation research   102(9): 1057-1064 (Journal)
Registered Authors
Mably, John, Xiong, Jing-Wei, Yu, Qingming, Zhang, Jiaojiao
Keywords
acyltransferase, hemangioblast, hematopoiesis, vasculogenesis, zebrafish, cloche
MeSH Terms
  • Acyltransferases/genetics
  • Acyltransferases/metabolism*
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/metabolism
  • Cell Lineage*/genetics
  • Down-Regulation
  • Embryonic Stem Cells/enzymology*
  • Endothelial Cells/enzymology*
  • Epistasis, Genetic
  • Gene Expression Regulation, Developmental
  • Gene Transfer Techniques
  • Genotype
  • Hematopoietic Stem Cells/enzymology*
  • Mice
  • Morpholines/metabolism
  • Mutation
  • Oligonucleotides, Antisense/metabolism
  • Phenotype
  • Proto-Oncogene Proteins/metabolism
  • RNA, Messenger/metabolism
  • Recombinant Fusion Proteins/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed
18388326 Full text @ Circ. Res.
Abstract
Hematopoietic and endothelial cells develop from a common progenitor, the hemangioblast, or directly from mesodermal cells. The molecular pathway that regulates the specification of both cell lineages remains elusive. Here, we show that a lysocardiolipin acyltransferase, lycat, is critical for the establishment of both hematopoietic and endothelial lineages. We isolated lycat from the deletion interval of cloche, a zebrafish mutant that has dramatically reduced hematopoietic and endothelial cell lineages. Reduction of lycat mRNA levels in wild-type zebrafish embryos decreases both endothelial and hematopoietic lineages. lycat mRNA rescues blood lineages in zebrafish cloche mutant embryos. E165R and G166L mutations in the highly conserved catalytic domain in Lycat abolish its function in zebrafish hematopoiesis. Epistasis analysis supports that lycahematopoietic lineages. lycat mRNA rescues blood lineages in zebrafish cloche mutant embryos. E165R and G166L mutations in the highly conserved catalt acts upstream of scl and etsrp in zebrafish hemangioblast development. These data indicate that lycat is the earliest known player in the generation of both endothelial and hematopoietic lineages.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes