PUBLICATION

Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos

Authors

Li, W.M., Webb, S.E., Chan, C.M., and Miller, A.L.

ID

ZDB-PUB-080306-37

Date

2008

Source

Developmental Biology 316(2): 228-248 (Journal)

Registered Authors

Miller, Andrew L., Webb, Sarah E.

Keywords

none

MeSH Terms

Calcium/physiology*
Genes, Reporter
Cytokinesis/physiology*
Actins/metabolism
Animals
Recombinant Fusion Proteins/metabolism
Embryo, Nonmammalian/cytology
Embryo, Nonmammalian/physiology*
Kinetics
Immunohistochemistry
Plasmids
Embryonic Development
Zebrafish/embryology*

PubMed

18313658 Full text @ Dev. Biol.

Abstract

The generation of a required series of localized Ca(2+) transients during cytokinesis in zebrafish embryos suggests that Ca(2+) plays a necessary role in regulating this process. Here, we report that cortical actin remodeling, characterized by the reorganization of the contractile band and the formation during furrow deepening of pericleavage F-actin enrichments (PAEs), requires a localized increase in intracellular Ca(2+), which is released from IP(3)-sensitive stores. We demonstrate that VAMP-2 vesicle fusion at the deepening furrow also requires Ca(2+) released via IP(3) receptors, as well as the presence of PAEs and the action of calpains. Finally, by expressing a dominant-negative form of the kinesin-like protein, kif23, we demonstrate that its recruitment to the furrow region is required for VAMP-2 vesicle transport; and via FRAP analysis, that kif23 localization is also Ca(2+)-dependent. Collectively, our data demonstrate that a localized increase in intracellular Ca(2+) is involved in regulating several key events during furrow deepening and subsequent apposition.

Genes / Markers

Figures