PUBLICATION
Molecular cloning and developmental expression of plakophilin 2 in zebrafish
- Authors
- Moriarty, M.A., Martin, E.D., Byrnes, L., and Grealy, M.
- ID
- ZDB-PUB-080102-9
- Date
- 2008
- Source
- Biochemical and Biophysical Research Communications 367(1): 124-129 (Journal)
- Registered Authors
- Byrnes, Lucy
- Keywords
- Plakophilin 2, Zebrafish, Desmosomes, Armadillo proteins, Development
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Adhesion/genetics
- Cell Adhesion/physiology
- Cells, Cultured
- Cloning, Molecular
- DNA, Complementary/genetics
- DNA, Complementary/metabolism
- Desmosomes/metabolism
- Gene Expression Regulation, Developmental/genetics*
- Gene Expression Regulation, Developmental/physiology
- Molecular Sequence Data
- Myocardium/metabolism
- Plakophilins/chemistry
- Plakophilins/genetics*
- Plakophilins/metabolism
- Zebrafish*/embryology
- Zebrafish*/genetics
- Zebrafish*/metabolism
- PubMed
- 18164260 Full text @ Biochem. Biophys. Res. Commun.
Citation
Moriarty, M.A., Martin, E.D., Byrnes, L., and Grealy, M. (2008) Molecular cloning and developmental expression of plakophilin 2 in zebrafish. Biochemical and Biophysical Research Communications. 367(1):124-129.
Abstract
Armadillo proteins are involved in providing strength and support to cells and tissues, nuclear transport, and transcriptional activation. In this report, we describe the identification and characterisation of the cDNA of the desmosomal armadillo protein plakophilin 2 in zebrafish. The 2448bp coding sequence encodes a predicted 815 amino acid protein, with nine armadillo repeats characteristic of the p120-catenin subfamily. It shares conserved N-glycosylation, myristoylation, and glycogen synthase kinase 3, casein kinase 2, and protein kinase C phosphorylation sites with mammalian armadillo proteins including plakoglobin and beta-catenin. Semi-quantitative reverse transcription polymerase chain reaction and whole mount in situ hybridisation show that it is expressed both maternally and zygotically. It is ubiquitously expressed during blastula stages but becomes restricted to epidermal and cardiac tissue during gastrulation. These results provide evidence that zebrafish plakophilin 2 is developmentally regulated with potential roles in cell adhesion, signalling, and cardiac and skin development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping