PUBLICATION

Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation

Authors
Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi, Y., Heath, J.K., and Perkins, A.C.
ID
ZDB-PUB-071227-13
Date
2008
Source
Developmental Biology   314(1): 12-22 (Journal)
Registered Authors
Heath, Joan K., Kikuchi, Yutaka, Mizoguchi, Takamasa, Verkade, Heather, Wilkins, Simon
Keywords
Epiboly, Mtx2, Mxtx2, Gastrulation, Morphogenetic movements, Yolk syncytial layer (YSL), Zebrafish, Endoderm, Microfilaments, F-actin
MeSH Terms
  • Actins/physiology*
  • Animals
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Embryo, Nonmammalian
  • DNA-Binding Proteins/genetics
  • DNA-Binding Proteins/physiology
  • Membrane Proteins/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Promoter Regions, Genetic
  • Gastrulation/physiology
  • SOXF Transcription Factors
  • Transcription Factors/genetics
  • Transcription Factors/physiology
  • High Mobility Group Proteins/genetics
  • High Mobility Group Proteins/physiology
  • Actin Cytoskeleton/physiology*
  • Cell Movement/physiology
(all 19)
PubMed
18154948 Full text @ Dev. Biol.
Abstract
The homeobox transcription factor Mtx2 is essential for epiboly, the first morphogenetic movement of gastrulation in zebrafish. Morpholino knockdown of Mtx2 results in stalling of epiboly and lysis due to yolk rupture. However, the mechanism of Mtx2 action is unknown. The role of mtx2 is surprising as most mix/bix family genes are thought to have roles in mesendoderm specification. Using a transgenic sox17-promoter driven EGFP line, we show that Mtx2 is not required for endoderm specification but is required for correct morphogenetic movements of endoderm and axial mesoderm. During normal zebrafish development, mtx2 is expressed at both the blastoderm margin and in the zebrafish equivalent of visceral endoderm, the extra-embryonic yolk syncytial layer (YSL). We show that formation of the YSL is not Mtx2 dependent, but that Mtx2 directs spatial arrangement of YSL nuclei. Furthermore, we demonstrate that Mtx2 knockdown results in loss of the YSL F-actin ring, a microfilament structure previously shown to be necessary for epiboly progression. In summary, we propose that Mtx2 acts within the YSL to regulate morphogenetic movements of both embryonic and extra-embryonic tissues, independently of cell fate specification.
Genes / Markers
Figures
Figure Gallery (8 images)
Show all Figures
Expression
Phenotype
Fish Conditions Stage Phenotype Figure
WT + MO1-mxtx2standard conditionsShield
WT + MO1-mxtx2standard conditionsShield
WT + MO1-mxtx2standard conditionsShield
WT + MO1-mxtx2standard conditionsShield
WT + MO1-mxtx2standard conditionsShield to 75%-epiboly
WT + MO1-mxtx2standard conditionsShield to 75%-epiboly
WT + MO1-mxtx2standard conditionsShield to 75%-epiboly
WT + MO1-mxtx2standard conditionsShield to Bud
WT + MO1-mxtx2standard conditions30%-epiboly
WT + MO1-mxtx2standard conditionsShield
1 - 10 of 24
Show
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
ha01TgTransgenic Insertion
    1 - 1 of 1
    Show
    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    Target Reagent Reagent Type
    mxtx2MO1-mxtx2MRPHLNO
    1 - 1 of 1
    Show
    Fish
    Fish
    ha01Tg
    ha01Tg + MO1-mxtx2
    WT
    WT + MO1-mxtx2
    1 - 4 of 4
    Show
    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
    Show
    Mapping
    No data available
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    Citation
    Wilkins, S.J., Yoong, S., Verkade, H., Mizoguchi, T., Plowman, S.J., Hancock, J.F., Kikuchi, Y., Heath, J.K., and Perkins, A.C. (2008) Mtx2 directs zebrafish morphogenetic movements during epiboly by regulating microfilament formation. Developmental Biology. 314(1):12-22.