PUBLICATION
Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish
- Authors
- Rieger, S., Volkmann, K., and Köster, R.W.
- ID
- ZDB-PUB-071227-12
- Date
- 2008
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 237(1): 276-285 (Journal)
- Registered Authors
- Köster, Reinhard W., Rieger, Sandra, Volkmann, Katrin
- Keywords
- polysialyltransferase, PSA-NCAM, cell adhesion, zebrafish, cerebellum, neuronal migration, bio-imaging
- MeSH Terms
-
- Reverse Transcriptase Polymerase Chain Reaction
- Animals
- Sialic Acids/metabolism
- Brain/embryology
- Brain/growth & development
- Brain/metabolism
- Cerebellum/embryology
- Cerebellum/growth & development
- Cerebellum/metabolism
- Immunohistochemistry
- Cell Movement/genetics*
- Cell Movement/physiology
- Neurons/cytology
- Neurons/enzymology
- Neurons/metabolism*
- Central Nervous System/embryology
- Central Nervous System/growth & development
- Central Nervous System/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- Zebrafish Proteins/physiology
- Neural Cell Adhesion Molecules/genetics
- Neural Cell Adhesion Molecules/metabolism
- Neural Cell Adhesion Molecules/physiology
- Gene Expression Regulation, Developmental
- Cell Adhesion/genetics
- Cell Adhesion/physiology
- Zebrafish/embryology
- Zebrafish/genetics*
- Zebrafish/growth & development
- Sialyltransferases/genetics*
- Sialyltransferases/metabolism
- PubMed
- 18095350 Full text @ Dev. Dyn.
Citation
Rieger, S., Volkmann, K., and Köster, R.W. (2008) Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(1):276-285.
Abstract
Modulation of cell-cell adhesion is crucial for regulating neuronal migration and maintenance of structural plasticity in the embryonic and mature brain. Such modulation can be obtained by the enzymatic attachment of polysialic acid (PSA) to the neural cell adhesion molecule (NCAM) by means of the polysialyltransferases STX and PST. Thus, differential expression of STX and PST is likely to be responsible for varying functions of PSA-NCAM during neuronal differentiation, maintenance, plasticity, and regeneration. We have isolated the zebrafish homologues of STX (St8sia2) and PST (St8sia4) and demonstrate that their expression in the embryonic and adult nervous system is often confined to regions of neuronal migration. Moreover, in the adult cerebellum, the complementary expression pattern of both polysialyltransferases suggests a function in regulating cerebellar neuronal plasticity. Enzymatic removal of PSA in the embryonic cerebellum results in impaired neuronal migration, suggesting that PSA-NCAM is a key regulator of motility for cerebellar neuronal progenitors.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping