PUBLICATION

Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish

Authors
Rieger, S., Volkmann, K., and Köster, R.W.
ID
ZDB-PUB-071227-12
Date
2008
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   237(1): 276-285 (Journal)
Registered Authors
Köster, Reinhard W., Rieger, Sandra, Volkmann, Katrin
Keywords
polysialyltransferase, PSA-NCAM, cell adhesion, zebrafish, cerebellum, neuronal migration, bio-imaging
MeSH Terms
  • Reverse Transcriptase Polymerase Chain Reaction
  • Brain/embryology
  • Brain/growth & development
  • Brain/metabolism
  • Cell Adhesion/genetics
  • Cell Adhesion/physiology
  • Cell Movement/genetics*
  • Cell Movement/physiology
  • Immunohistochemistry
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Zebrafish Proteins/physiology
  • Central Nervous System/embryology
  • Central Nervous System/growth & development
  • Central Nervous System/metabolism
  • Gene Expression Regulation, Developmental
  • Neural Cell Adhesion Molecules/genetics
  • Neural Cell Adhesion Molecules/metabolism
  • Neural Cell Adhesion Molecules/physiology
  • Animals
  • Cerebellum/embryology
  • Cerebellum/growth & development
  • Cerebellum/metabolism
  • Neurons/cytology
  • Neurons/enzymology
  • Neurons/metabolism*
  • Sialyltransferases/genetics*
  • Sialyltransferases/metabolism
  • Sialic Acids/metabolism
(all 32)
PubMed
18095350 Full text @ Dev. Dyn.
Abstract
Modulation of cell-cell adhesion is crucial for regulating neuronal migration and maintenance of structural plasticity in the embryonic and mature brain. Such modulation can be obtained by the enzymatic attachment of polysialic acid (PSA) to the neural cell adhesion molecule (NCAM) by means of the polysialyltransferases STX and PST. Thus, differential expression of STX and PST is likely to be responsible for varying functions of PSA-NCAM during neuronal differentiation, maintenance, plasticity, and regeneration. We have isolated the zebrafish homologues of STX (St8sia2) and PST (St8sia4) and demonstrate that their expression in the embryonic and adult nervous system is often confined to regions of neuronal migration. Moreover, in the adult cerebellum, the complementary expression pattern of both polysialyltransferases suggests a function in regulating cerebellar neuronal plasticity. Enzymatic removal of PSA in the embryonic cerebellum results in impaired neuronal migration, suggesting that PSA-NCAM is a key regulator of motility for cerebellar neuronal progenitors.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b2
    		Unknown
    rw0TgTransgenic Insertion
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      No data available
      Fish
      Fish
      AB
      brsb2
      rw0Tg
      1 - 3 of 3
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      Antibodies
      Name Type Antigen Genes Isotypes Host Organism
      Ab2-psamonoclonal
        		IgG2aMouse
        1 - 1 of 1
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        Orthology
        No data available
        Engineered Foreign Genes
        Marker Marker Type Name
        GFPEFGGFP
        1 - 1 of 1
        Show
        Mapping
        No data available
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        Citation
        Rieger, S., Volkmann, K., and Köster, R.W. (2008) Polysialyltransferase expression is linked to neuronal migration in the developing and adult zebrafish. Developmental Dynamics : an official publication of the American Association of Anatomists. 237(1):276-285.