PUBLICATION
Tol2: a versatile gene transfer vector in vertebrates
- Authors
- Kawakami, K.
- ID
- ZDB-PUB-071210-14
- Date
- 2007
- Source
- Genome biology 8(1): S7 (Review)
- Registered Authors
- Kawakami, Koichi
- Keywords
- none
- MeSH Terms
-
- Animals
- DNA Transposable Elements/genetics*
- Gene Transfer Techniques*
- Genetic Vectors*
- Germ Cells
- Vertebrates/genetics*
- PubMed
- 18047699 Full text @ Genome Biol.
Citation
Kawakami, K. (2007) Tol2: a versatile gene transfer vector in vertebrates. Genome biology. 8(1):S7.
Abstract
The medaka fish Tol2 element is an autonomous transposon that encodes a fully functional transposase. The transposase protein can catalyze transposition of a transposon construct that has 200 and 150 base pairs of DNA from the left and right ends of the Tol2 sequence, respectively. These sequences contain essential terminal inverted repeats and subterminal sequences. DNA inserts of fairly large sizes (as large as 11 kilobases) can be cloned between these sequences without reducing transpositional activity. The Tol2 transposon system has been shown to be active in all vertebrate cells tested thus far, including zebrafish, Xenopus, chicken, mouse, and human. In this review I describe and discuss how the Tol2 transposon is being applied to transgenic studies in these vertebrates, and possible future applications.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping