PUBLICATION

Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter

Authors
Wu, Y.L., Pan, X., Mudumana, S.P., Wang, H., Kee, P.W., and Gong, Z.
ID
ZDB-PUB-071129-4
Date
2008
Source
Gene   408(1-2): 85-94 (Journal)
Registered Authors
Gong, Zhiyuan, Mudumana, Sudha Puttur, Pan, Xiufang, Wang, Hai, Wu, Yi Lian
Keywords
Small heat shock protein, Hsp25, Arsenic, Heart, Muscle, Embryo
MeSH Terms
  • Molecular Sequence Data
  • Cloning, Molecular
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism
  • Animals
  • Heat-Shock Response/genetics*
  • HSP27 Heat-Shock Proteins
  • Animals, Genetically Modified*
  • Heat-Shock Proteins/genetics*
  • Heat-Shock Proteins/metabolism
  • Promoter Regions, Genetic*
  • Embryo, Nonmammalian/metabolism
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Green Fluorescent Proteins/genetics*
  • Green Fluorescent Proteins/metabolism
(all 17)
PubMed
18037593 Full text @ Gene
Abstract
In the present study, a zebrafish hsp27 promoter was isolated and used to develop heat shock inducible gfp transgenic zebrafish. The endogenous hsp27 mRNAs were constitutively expressed from 4 hpf and increased in several regions of brain, heart and somites in early embryogenesis until 24 hpf. Subsequently, the expression was reduced significantly but maintained in the heart and ears. Heat shock induced hsp27 mRNAs in the blastoderm from 6 hpf and later in somites, branchial arches and several regions of brain. Similarly in hsp27-gfp transgenic zebrafish, constitutive GFP expression was observed from 11 hpf. GFP expression was mainly in the skin cells and increased to the peak level at 7 dpf, followed by a reduction. The constitutive GFP expression in the heart was initiated from 50 hpf and maintained even in the adult fish. After heat shock, GFP expression was mainly induced in the muscle in addition to a mild increase in the skin and heart. The early stages of the embryos were more sensitive than late stages as the time required for induced GFP expression in the muscle is shorter. Thus, the hsp27-gfp transgenic line generally recapitulates the expression pattern and heat shock inducibility of endogenous hsp27 RNAs. We also tested the potential of using the hsp27-gfp transgenic zebrafish embryos for heavy metal induction and demonstrated the inducibility of GFP expression by arsenic; this pattern of induction was also supported by examination of endogenous hsp27 mRNA.
Genes / Markers
Figures
Figure Gallery (5 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
gz11TgTransgenic Insertion
    1 - 1 of 1
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    Human Disease / Model
    No data available
    Sequence Targeting Reagents
    No data available
    Fish
    Fish
    gz11Tg/+
    WT
    1 - 2 of 2
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    Antibodies
    No data available
    Orthology
    No data available
    Engineered Foreign Genes
    Marker Marker Type Name
    EGFPEFGEGFP
    1 - 1 of 1
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    Mapping
    No data available
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    Citation
    Wu, Y.L., Pan, X., Mudumana, S.P., Wang, H., Kee, P.W., and Gong, Z. (2008) Development of a heat shock inducible gfp transgenic zebrafish line by using the zebrafish hsp27 promoter. Gene. 408(1-2):85-94.