PUBLICATION
The zebrafish activating immune receptor Nitr9 signals via Dap12
- Authors
- Wei, S., Zhou, J.M., Chen, X., Shah, R.N., Liu, J., Orcutt, T.M., Traver, D., Djeu, J.Y., Litman, G.W., and Yoder, J.A.
- ID
- ZDB-PUB-071001-7
- Date
- 2007
- Source
- Immunogenetics 59(10): 813-821 (Journal)
- Registered Authors
- Litman, Gary W., Orcutt, Timothy, Shah, Radhika, Traver, David, Yoder, Jeff
- Keywords
- Natural cytotoxicity, DAP10, ERK, AKT, DAP12, NITR
- MeSH Terms
-
- Zebrafish
- Recombinant Proteins/immunology
- Recombinant Proteins/metabolism
- Signal Transduction*/immunology
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/immunology
- Adaptor Proteins, Signal Transducing/metabolism
- Sequence Alignment
- Amino Acid Sequence
- Animals
- Zebrafish Proteins/genetics
- Zebrafish Proteins/immunology
- Zebrafish Proteins/metabolism*
- Receptors, KIR/genetics
- Receptors, KIR/immunology
- Receptors, KIR/metabolism*
- Phosphorylation
- Molecular Sequence Data
- Humans
- Cell Line
- PubMed
- 17891481 Full text @ Immunogenetics
Citation
Wei, S., Zhou, J.M., Chen, X., Shah, R.N., Liu, J., Orcutt, T.M., Traver, D., Djeu, J.Y., Litman, G.W., and Yoder, J.A. (2007) The zebrafish activating immune receptor Nitr9 signals via Dap12. Immunogenetics. 59(10):813-821.
Abstract
Both inhibitory and activating forms of natural killer (NK) cell receptors are found in mammals. The activating receptors play a direct role in the recognition of virally infected or transformed cells and transduce activating signals into the cell by partnering with an adaptor protein, which contains a cytoplasmic activation motif. Activating NK receptors encoded by the mammalian leukocyte receptor complex (e.g., killer cell immunoglobulin-like receptors) and the natural killer complex (e.g., Ly49s) partner with the adaptor protein DAP12, whereas NK receptors encoded in the CD94/NKG2 complex partner with the adaptor protein DAP10. Novel immune-type receptors (NITRs) found in bony fish share several common features with immunoglobulin-type NK receptors. Nitr9 is a putative activating receptor in zebrafish that induces cytotoxicity within the context of human NK cells. One isoform of Nitr9, Nitr9L, is shown here to preferentially partner with a zebrafish ortholog of Dap12. Cross-linking the Nitr9L-Dap12 complex results in activation of the phosphytidylinositol 3-kinase-->AKT-->extracellular signal-regulated kinase pathway suggesting that the DAP12-based activating pathway is conserved between bony fish and mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping