PUBLICATION
The zebrafish activating immune receptor Nitr9 signals via Dap12
- Authors
- Wei, S., Zhou, J.M., Chen, X., Shah, R.N., Liu, J., Orcutt, T.M., Traver, D., Djeu, J.Y., Litman, G.W., and Yoder, J.A.
- ID
- ZDB-PUB-071001-7
- Date
- 2007
- Source
- Immunogenetics 59(10): 813-821 (Journal)
- Registered Authors
- Litman, Gary W., Orcutt, Timothy, Shah, Radhika, Traver, David, Yoder, Jeff
- Keywords
- Natural cytotoxicity, DAP10, ERK, AKT, DAP12, NITR
- MeSH Terms
-
- Amino Acid Sequence
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/immunology
- Adaptor Proteins, Signal Transducing/metabolism
- Signal Transduction*/immunology
- PubMed
- 17891481 Full text @ Immunogenetics
Abstract
Both inhibitory and activating forms of natural killer (NK) cell receptors are found in mammals. The activating receptors play a direct role in the recognition of virally infected or transformed cells and transduce activating signals into the cell by partnering with an adaptor protein, which contains a cytoplasmic activation motif. Activating NK receptors encoded by the mammalian leukocyte receptor complex (e.g., killer cell immunoglobulin-like receptors) and the natural killer complex (e.g., Ly49s) partner with the adaptor protein DAP12, whereas NK receptors encoded in the CD94/NKG2 complex partner with the adaptor protein DAP10. Novel immune-type receptors (NITRs) found in bony fish share several common features with immunoglobulin-type NK receptors. Nitr9 is a putative activating receptor in zebrafish that induces cytotoxicity within the context of human NK cells. One isoform of Nitr9, Nitr9L, is shown here to preferentially partner with a zebrafish ortholog of Dap12. Cross-linking the Nitr9L-Dap12 complex results in activation of the phosphytidylinositol 3-kinase-->AKT-->extracellular signal-regulated kinase pathway suggesting that the DAP12-based activating pathway is conserved between bony fish and mammals.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping