PUBLICATION
The protein tyrosine phosphatase Pez regulates TGF{beta}, epithelial mesenchymal transition, and organ development
- Authors
- Wyatt, L., Wadham, C., Crocker, L.A., Lardelli, M., and Khew-Goodall, Y.
- ID
- ZDB-PUB-071001-19
- Date
- 2007
- Source
- The Journal of cell biology 178(7): 1223-1235 (Journal)
- Registered Authors
- Lardelli, Michael
- Keywords
- none
- MeSH Terms
-
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Transforming Growth Factor beta3/genetics
- Transforming Growth Factor beta3/metabolism
- Humans
- Phenotype
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Transforming Growth Factor beta1/genetics
- Transforming Growth Factor beta1/metabolism
- Transcription Factors/genetics
- Transcription Factors/metabolism
- Organogenesis*/drug effects
- Transforming Growth Factor beta2/genetics
- Transforming Growth Factor beta2/metabolism
- Cell Nucleus/metabolism
- RNA, Messenger/genetics
- RNA, Messenger/metabolism
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/metabolism
- Protein Tyrosine Phosphatases/genetics
- Protein Tyrosine Phosphatases/metabolism*
- Dogs
- Oligonucleotides, Antisense/pharmacology
- Epithelial Cells/cytology*
- Epithelial Cells/drug effects
- Epithelial Cells/metabolism
- Embryonic Development/drug effects
- Signal Transduction/drug effects
- Mesoderm/cytology*
- Mesoderm/drug effects
- Mesoderm/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Gene Expression Regulation, Developmental/drug effects
- Protein Transport/drug effects
- Animals
- Smad4 Protein/metabolism
- Protein Tyrosine Phosphatases, Non-Receptor/genetics
- Protein Tyrosine Phosphatases, Non-Receptor/metabolism
- PubMed
- 17893246 Full text @ J. Cell Biol.
Citation
Wyatt, L., Wadham, C., Crocker, L.A., Lardelli, M., and Khew-Goodall, Y. (2007) The protein tyrosine phosphatase Pez regulates TGF{beta}, epithelial mesenchymal transition, and organ development. The Journal of cell biology. 178(7):1223-1235.
Abstract
Epithelial-mesenchymal transition (EMT), crucial during embryogenesis for new tissue and organ formation, is also considered to be a prerequisite to cancer metastasis. We report here that the protein tyrosine phosphatase Pez is expressed transiently in discrete locations in developing brain, heart, pharyngeal arches, and somites in zebrafish embryos. We also find that Pez knock-down results in defects in these organs, indicating a crucial role in organogenesis. Overexpression of Pez in epithelial MDCK cells causes EMT, with a drastic change in cell morphology and function that is accompanied by changes in gene expression typical of EMT. Transfection of Pez induced TGFbeta signaling, critical in developmental EMT with a likely role also in oncogenic EMT. In zebrafish, TGFbeta3 is co- expressed with Pez in a number of tissues and its expression was lost from these tissues when Pez expression was knocked down. Together, our data suggest Pez plays a crucial role in organogenesis by inducing TGFbeta and EMT.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping