ZFIN ID: ZDB-PUB-070907-30
Even fluorescence excitation by multidirectional selective plane illumination microscopy (mSPIM)
Huisken, J., and Stainier, D.Y.
Date: 2007
Source: Optics letters 32(17): 2608-2610 (Journal)
Registered Authors: Stainier, Didier
Keywords: none
MeSH Terms: Absorption; Animals; Embryo, Nonmammalian/pathology; Equipment Design; Image Processing, Computer-Assisted (all 13) expand
PubMed: 17767321 Full text @ Opt. Lett.
ABSTRACT
Multidirectional selective plane illumination microscopy (mSPIM) reduces absorption and scattering artifacts and provides an evenly illuminated focal plane. mSPIM solves two common problems in light-sheet-based imaging techniques: The shadowing in the excitation path due to absorption in the specimen is eliminated by pivoting the light sheet; the spread of the light sheet by scattering in the sample is compensated by illuminating the sample consecutively from opposing directions. The resulting two images are computationally fused yielding a superior image. The effective light sheet is thinner, and the axial resolution is increased by square root 2 over single-directional SPIM. The multidirectional illumination proves essential in biological specimens such as millimeter-sized embryos. The performance of mSPIM is demonstrated by the imaging of live zebrafish embryos.
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