PUBLICATION

A role for the Myoblast city homologues Dock1 and Dock5 and the adaptor proteins Crk and Crk-like in zebrafish myoblast fusion

Authors
Moore, C.A., Parkin, C.A., Bidet, Y., and Ingham, P.W.
ID
ZDB-PUB-070806-27
Date
2007
Source
Development (Cambridge, England)   134(17): 3145-3153 (Journal)
Registered Authors
Bidet, Yannick, Ingham, Philip, Moore, Catherine, Parkin, Caroline
Keywords
Crk, Crkl, DOCK1, DOCK5, Myoblast city, Myoblast fusion, Zebrafish
MeSH Terms
  • Embryo, Nonmammalian
  • Cell Fusion
  • Drosophila Proteins/genetics
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
  • Nuclear Proteins/physiology*
  • Sequence Homology, Amino Acid
  • Animals
  • Cloning, Molecular
  • Muscle Fibers, Fast-Twitch/cytology
  • Muscle Development/genetics*
  • Cytoskeletal Proteins/genetics
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/physiology
  • Oncogene Protein v-crk/physiology*
  • Adaptor Proteins, Signal Transducing/physiology*
  • Myoblasts, Skeletal/physiology
  • rac GTP-Binding Proteins
(all 19)
PubMed
17670792 Full text @ Development
Abstract
Myoblast fusion follows a defined sequence of events that is strikingly similar in vertebrates and invertebrates. Genetic analysis in Drosophila has identified many of the molecules that mediate the different steps in the fusion process; by contrast, the molecular basis of myoblast fusion during vertebrate embryogenesis remains poorly characterised. A key component of the intracellular fusion pathway in Drosophila is the protein encoded by the myoblast city (mbc) gene, a close homologue of the vertebrate protein dedicator of cytokinesis 1 (DOCK1, formerly DOCK180). Using morpholino antisense-oligonucleotide-mediated knockdown of gene activity in the zebrafish embryo, we show that the fusion of embryonic fast-twitch myoblasts requires the activities of Dock1 and the closely related Dock5 protein. In addition, we show that the adaptor proteins Crk and Crk-like (Crkl), with which Dock proteins are known to interact physically, are also required for myoblast fusion.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
crkMO1-crkMRPHLNO
crkMO2-crkMRPHLNO
crklMO1-crklMRPHLNO
crklMO2-crklMRPHLNO
dock1MO2-dock1MRPHLNO
dock1MO1-dock1MRPHLNO
dock5MO1-dock5MRPHLNO
dock5MO2-dock5MRPHLNO
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Fish
Antibodies
No data available
Orthology
Gene Orthology
dock1
dock5
1 - 2 of 2
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Engineered Foreign Genes
No data available
Mapping