BMPER is a conserved regulator of hematopoietic and vascular development in zebrafish

Moser, M., Yu, Q., Bode, C., Xiong, J.W., and Patterson, C.
Journal of Molecular and Cellular Cardiology   43(3): 243-253 (Journal)
Registered Authors
Xiong, Jing-Wei, Yu, Qingming
Developmental biology, Gene expression, Blood vessel formation, Hematopoiesis, Zebrafish, Bone morphogenetic protein, BMPER
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Animals, Genetically Modified
  • Blood Vessels/embryology*
  • Carrier Proteins/genetics*
  • Carrier Proteins/physiology
  • Cloning, Molecular
  • Embryo, Nonmammalian
  • GTPase-Activating Proteins/genetics
  • GTPase-Activating Proteins/physiology*
  • Gene Expression Regulation, Developmental*
  • Green Fluorescent Proteins/metabolism
  • Hematopoiesis/genetics*
  • Hematopoiesis/physiology
  • In Situ Hybridization
  • Molecular Sequence Data
  • Phylogeny
  • Protein Structure, Tertiary
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Sequence Homology, Amino Acid
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/physiology*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
17618647 Full text @ J. Mol. Cell. Cardiol.
For the proper development of vertebrate embryos as well as for survival of the adult organism, it is essential to form a functional vascular system. Molecules involved in this process are members of highly conserved families of proteins that exert conserved functions across species. Bone morphogenetic proteins (BMP) are extracellular factors that are regulated by extracellular modulators and bind to BMP receptors, which in turn activate intracellular signaling cascades. BMPs are necessary not only for induction of endothelial and hematopoietic lineages but also for further endothelial and hematopoietic cell differentiation. Previously, we identified BMPER (BMP endothelial cell precursor derived regulator) and demonstrated its spatiotemporal expression at sites of vasculogenesis and direct modulation of BMP activity. To directly investigate the role of BMPER in vascular development, we cloned the BMPER ortholog in zebrafish (zbmper). It is expressed at sites of high BMP activity, including vascular precursor cells located in the aortic arches and the intermediate cell mass during zebrafish embryonic development. Knockdown of zbmper results in a dorsalized phenotype, a reduced number of gata1 expressing hematopoietic precursor cells and of circulating blood cells as well as in a vascular phenotype. The generation of the caudal vein is compromised and the pattern guiding of the intersomitic vessels is disturbed, indicating that zbmper is required for early steps in vascular pattern formation and hematopoiesis in zebrafish.
Genes / Markers
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Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes