PUBLICATION

Zebrafish dou yan mutation causes patterning defects and extensive cell death in the retina

Authors

Catalano, A.E., Raymond, P.A., Goldman, D., and Wei, X.

ID

ZDB-PUB-070427-17

Date

2007

Source

Developmental Dynamics : an official publication of the American Association of Anatomists 236(5): 1295-1306 (Journal)

Registered Authors

Goldman, Dan, Raymond, Pamela, Wei, Xiangyun

Keywords

retina, dou yan, retinal lamination, cell death, small eye

MeSH Terms

Cell Proliferation
Animals
Phenotype
Brain/embryology
Organ Size/genetics
Eye Abnormalities/embryology
Eye Abnormalities/genetics
Eye Abnormalities/pathology
Zebrafish/embryology*
Zebrafish/genetics*
Retina/abnormalities*
Retina/embryology
Retina/pathology
Female
Mutation*
Male
Cell Death

(all 17)

PubMed

17436278 Full text @ Dev. Dyn.

Abstract

The size of an organ is largely determined by the number of cells it contains, which in turn is regulated by two opposing processes, cell proliferation and cell death, however, it is generally not clear how cell proliferation and cell death are coordinated during development. Here, we characterize the zebrafish dou yan(mi234) mutation that results in a dramatic reduction of retinal size and a disruption of retinal differentiation and lamination. The retinal size reduction is caused by increased retinal cell death in a non-cell-autonomous manner during early development. The phenotypic defect in dou yan(mi234) arises coincident with the onset of retinal neurogenesis and differentiation. Interestingly, unlike many other small eye mutations, the mutation does not increase the level of cell death in the brain, suggesting that the brain and retina use different mechanisms to maintain cell survival. Identification and further study of the dou yan gene will enhance our understanding of the molecular mechanisms regulating retinal cellular homeostasis, i.e., the balance between cell proliferation and cell death.

Genes / Markers

Figures

Show all Figures

Expression

Fish	Conditions	Stage	Anatomy	Assay	Figure
doy^mi234/mi234	standard conditions	Day 5	retinal ganglion cell	IHC	Fig. 7
doy^mi234/mi234	standard conditions	Protruding-mouth	retinal ganglion cell	IHC	Fig. 8
WT	standard conditions	Day 5	retinal ganglion cell	IHC	Fig. 7
WT	standard conditions	Protruding-mouth	retinal ganglion cell	IHC	Fig. 8
doy^mi234/mi234	standard conditions	Protruding-mouth	Muller cell	IHC	Fig. 8
doy^mi234/mi234	standard conditions	Day 5	Muller cell	IHC	Fig. 7
WT	standard conditions	Protruding-mouth	Muller cell	IHC	Fig. 8
WT	standard conditions	Day 5	Muller cell	IHC	Fig. 7
doy^mi234/mi234	standard conditions	Day 5	retinal bipolar neuron	IHC	Fig. 7
doy^mi234/mi234	standard conditions	Protruding-mouth	retinal bipolar neuron	IHC	Fig. 8

1 - 10 of 18

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Phenotype

Fish	Conditions	Stage	Phenotype	Figure
doy^mi234/mi234	standard conditions	Long-pec	eye decreased size, abnormal	Fig. 1
doy^mi234/mi234	standard conditions	Long-pec	lens decreased size, abnormal	Fig. 2
doy^mi234/mi234	standard conditions	Long-pec	retina decreased size, abnormal	Fig. 2
doy^mi234/mi234	standard conditions	Long-pec	whole organism increased length, abnormal	Fig. 1
doy^mi234/mi234	standard conditions	Protruding-mouth	retina decreased size, abnormal	Fig. 2
doy^mi234/mi234	standard conditions	Protruding-mouth	retina layer formation disrupted, abnormal	Fig. 2
doy^mi234/mi234	standard conditions	Day 4	lens decreased size, abnormal	Fig. 2
doy^mi234/mi234	standard conditions	Day 4	optic tectum decreased size, abnormal	Fig. 3
doy^mi234/mi234	standard conditions	Day 4	retina decreased size, abnormal	Fig. 2
doy^mi234/mi234	standard conditions	Day 4	retina layer formation disrupted, abnormal	Fig. 2

1 - 10 of 15

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Mutations / Transgenics

Allele	Construct	Type	Affected Genomic Region
mi234		Point Mutation	doy

Human Disease / Model

Sequence Targeting Reagents

Fish

Fish
doy^mi234/mi234
WT

Antibodies

Orthology

Engineered Foreign Genes

Mapping

Entity Type	Entity Symbol	Location
GENE	doy	Chr: 21 Details
SSLP	z11532	Chr: 21 Details
SSLP	z26044	Chr: 21 Details

Catalano et al., 2007 ZDB-PUB-070427-17 PMID:17436278

Zebrafish dou yan mutation causes patterning defects and extensive cell death in the retina

Catalano et al., 2007

ZDB-PUB-070427-17
PMID:17436278