ZFIN ID: ZDB-PUB-070330-18
Targeting neural circuitry in zebrafish using GAL4 enhancer trapping
Scott, E.K., Mason, L., Arrenberg, A.B., Ziv, L., Gosse, N.J., Xiao, T., Chi, N.C., Asakawa, K., Kawakami, K., and Baier, H.
Date: 2007
Source: Nature Methods   4(4): 323-326 (Journal)
Registered Authors: Arrenberg, Aristides, Baier, Herwig, Gosse, Nathan, Kawakami, Koichi, Mason, Lindsay, Scott, Ethan, Xiao, Tong, Ziv, Limor
Keywords: none
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Brain*/cytology
  • Brain*/embryology
  • Brain*/metabolism
  • DNA-Binding Proteins
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation, Developmental*
  • Humans
  • Neurons*/metabolism
  • Pilot Projects
  • Saccharomyces cerevisiae Proteins/biosynthesis
  • Saccharomyces cerevisiae Proteins/genetics*
  • Transcription Factors/biosynthesis
  • Transcription Factors/genetics*
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed: 17369834 Full text @ Nat. Methods
We present a pilot enhancer trap screen using GAL4 to drive expression of upstream activator sequence (UAS)-linked transgenes in expression patterns dictated by endogenous enhancers in zebrafish. The patterns presented include expression in small subsets of neurons throughout the larval brain, which in some cases persist into adult. Through targeted photoconversion of UAS-driven Kaede and variegated expression of UAS-driven GFP in single cells, we begin to characterize the cellular components of labeled circuits.