ZFIN ID: ZDB-PUB-061227-4
Expression analysis of the family of 14-3-3 proteins in zebrafish development
Besser, J., Bagowski, C.P., Salas-Vidal, E., van Hemert, M.J., Bussmann, J., and Spaink, H.P.
Date: 2007
Source: Gene expression patterns : GEP   7(4): 511-520 (Journal)
Registered Authors: Bagowski, Christoph P., Bussmann, Jeroen, Salas-Vidal, Enrique, Spaink, Herman P.
Keywords: 14-3-3 gene family, Danio rerio zebrafish embryogenesis expression patterns, Genomic location, Phylogenetic tree, Whole mount in situ hybridization, Microarray analysis, Ywha genes
MeSH Terms:
  • 14-3-3 Proteins/genetics*
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development
  • Gene Expression*
  • Humans
  • Oligonucleotide Array Sequence Analysis
  • Phylogeny
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
PubMed: 17127101 Full text @ Gene Expr. Patterns
14-3-3 proteins comprise a family of dimeric multi-functional proteins present in all eukaryotes, that are important in a whelm of ubiquitous biological processes. We have analyzed the genomic structure of all 14-3-3s from zebrafish comprising 11 genes and have analyzed their phylogeny. The gene family was cloned and its expression pattern in zebrafish embryogenesis was analyzed by whole mount in situ hybridization and microarray analysis with gene specific probes. We demonstrate that maternal mRNA of 14-3-3s is expressed evenly at the first cell division. At later stage all genes are expressed in a patterned way with, in most cases, intricate patterns in the developing brain. Our result shows distinct expression patterns of various genes. Microarray results show that differences in expression levels of highly similar 14-3-3 genes also occur in the adult stage.