PUBLICATION

Essential role for Csk upstream of Fyn and Yes in zebrafish gastrulation

Authors
Jopling, C., and den Hertog, J.
ID
ZDB-PUB-061227-31
Date
2007
Source
Mechanisms of Development   124(2): 129-136 (Journal)
Registered Authors
den Hertog, Jeroen, Jopling, Chris
Keywords
Gastrulation, Cell movements, Csk, Src family kinases, Zebrafish
MeSH Terms
none
PubMed
17157484 Full text @ Mech. Dev.
Abstract
Morphogenetic cell movements during gastrulation shape the vertebrate embryo bodyplan. Non-canonical Wnt signaling has been established to regulate convergence and extension cell movements that mediate anterior-posterior axis elongation. In recent years, many other factors have been implicated in the process by modulation of non-canonical Wnt signaling or by different, unknown mechanisms. We have found that the Src family kinases, Fyn and Yes, are required for normal convergence and extension cell movements in zebrafish embryonic development and they signal in parallel to non-canonical Wnts, eventually converging on a common downstream factor, RhoA. Here, we report that Csk, a negative regulator of Src family kinases has a role in gastrulation cell movements as well. Csk knock down induced a phenotype that was similar to the defects observed after knock down of Fyn and Yes, in that gastrulation cell movements were impaired, without affecting cell fate. The Csk knock down phenotype was rescued by simultaneous partial knock down of Fyn and Yes. We conclude that Csk acts upstream of Fyn and Yes to control vertebrate gastrulation cell movements.
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