PUBLICATION
Heat shock factor 1 is required for constitutive Hsp70 expression and normal lens development in embryonic zebrafish
- Authors
- Evans, T.G., Belak, Z., Ovsenek, N., and Krone, P.H.
- ID
- ZDB-PUB-061227-12
- Date
- 2007
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 146(1): 131-140 (Journal)
- Registered Authors
- Evans, Tyler, Krone, Patrick H.
- Keywords
- Zebrafish embryo, Heat shock protein 70, Heat shock factor, Lens development, Morpholino
- MeSH Terms
-
- Morpholines/pharmacology
- Embryo, Nonmammalian/physiology*
- Oligodeoxyribonucleotides, Antisense/pharmacology
- HSP70 Heat-Shock Proteins/biosynthesis*
- Animals
- Lens, Crystalline/embryology*
- Gene Expression Regulation, Developmental
- Phenotype
- Heat-Shock Proteins/physiology*
- Zebrafish/embryology*
- Zebrafish/physiology
- Electrophoretic Mobility Shift Assay
- PubMed
- 17134927 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Evans, T.G., Belak, Z., Ovsenek, N., and Krone, P.H. (2007) Heat shock factor 1 is required for constitutive Hsp70 expression and normal lens development in embryonic zebrafish. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 146(1):131-140.
Abstract
Heat shock factors (HSFs) are the major transcription factors responsible for heat-induced upregulation of heat shock protein (Hsp) genes. All three mammalian HSFs (HSF1, HSF2, HSF4) have also been shown to be required for normal mammalian development. It is currently unknown if HSFs play similarly important roles during normal development of non-mammalian vertebrates. In the present study, a morpholino modified antisense oligonucleotide (MO) approach targeted against hsf1 mRNA (hsf1-MO) was used to examine the requirement of HSF1 in zebrafish development. Embryos depleted of HSF1 displayed a reproducible small eye phenotype characterized by an immature lens and a disorganized retinal structure. These defects were strikingly similar to those observed when constitutive, lens specific Hsp70 expression was reduced through the microinjection of MO targeting hsp70. The data suggest that HSF1 is involved in regulating constitutive lens specific expression of hsp70 in the embryonic zebrafish. This conclusion is supported by a marked reduction in Hsp70 protein in hsf1-MO injected embryos. Microinjection of MO targeted to hsf2 mRNA (hsf2-MO) did not result in a small eye phenotype in a significant number of embryos. These data also suggest that HSF1 and HSF2 play distinct roles in non-mammalian vertebrates, similarly to what has been demonstrated previously in mouse.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping