PUBLICATION
Sequestration of retinyl esters is essential for retinoid signaling in the zebrafish embryo
- Authors
- Isken, A., Holzschuh, J., Lampert, J.M., Fischer, L., Oberhauser, V., Palczewski, K., and von Lintig, J.
- ID
- ZDB-PUB-061116-13
- Date
- 2007
- Source
- The Journal of biological chemistry 282(2): 1144-1151 (Journal)
- Registered Authors
- Holzschuh, Jochen, Lampert, Johanna
- Keywords
- none
- MeSH Terms
-
- Acyltransferases/genetics
- Acyltransferases/metabolism
- Aldehyde Oxidoreductases/genetics
- Aldehyde Oxidoreductases/metabolism
- Amino Acid Sequence
- Animals
- Cell Line
- Drosophila
- Egg Yolk/metabolism
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Esters/metabolism
- Eye/embryology*
- Eye/metabolism*
- Gene Expression Regulation, Developmental
- Homeostasis/physiology
- Molecular Sequence Data
- Retinaldehyde/metabolism
- Retinoids/metabolism*
- Signal Transduction/physiology*
- Tretinoin/metabolism
- Vitamin A/metabolism
- Zebrafish/embryology*
- Zebrafish/metabolism*
- PubMed
- 17098734 Full text @ J. Biol. Chem.
Citation
Isken, A., Holzschuh, J., Lampert, J.M., Fischer, L., Oberhauser, V., Palczewski, K., and von Lintig, J. (2007) Sequestration of retinyl esters is essential for retinoid signaling in the zebrafish embryo. The Journal of biological chemistry. 282(2):1144-1151.
Abstract
For vertebrate development, vitamin A (all-trans retinol) is required in quantitative different amounts and spatiotemporal distribution for the production of retinoic acid, a nuclear hormone receptor ligand, and 11-cis retinal, the chromophore of visual pigments. We show here for zebrafish that embryonic retinoid homeostasis essentially depends on the activity of a lecithin:retinol acyltransferase (Lratb). During embryogenesis, lratb is expressed in mostly non-overlapping domains opposite to retinal dehydrogenase 2 (raldh2), the key enzyme for retinoic acid synthesis. Blocking retinyl ester formation by a targeted knock down of Lratb results in a significant increased retinoic acid levels, which lead to severe embryonic patterning defects. Thus, we provide evidence that a balanced competition between Lratb and Raldh2 for yolk vitamin A defines embryonic compartments either for retinyl ester or retinoic acid synthesis. This homeostatic mechanism dynamically adjusts embryonic retinoic acid levels for gene regulation, concomitantly sequestering excess yolk vitamin A in the form of retinyl esters for the establishment of larval vision later during development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping