ZFIN ID: ZDB-PUB-061108-23
Novel transcript nort is a downstream target gene of the Notch signaling pathway in zebrafish
Tsutsumi, M., and Itoh, M.
Date: 2007
Source: Gene expression patterns : GEP   793): 227-232 (Journal)
Registered Authors: Itoh, Motoyuki
Keywords: Notch signaling pathway, Neural plate, Noncoding RNA, Zebrafish, Danio rerio, Microarray, her4, Su(H), elavl3, SPS, Suppressor of Hairless paired binding site, notch1a, notch3, mind bomb, nort
MeSH Terms:
  • 5' Flanking Region/genetics
  • Animals
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental*
  • Nervous System/embryology
  • Nervous System/metabolism
  • Oligonucleotide Array Sequence Analysis
  • RNA, Untranslated/genetics*
  • RNA, Untranslated/metabolism
  • Receptors, Notch/metabolism*
  • Signal Transduction*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
  • Zebrafish Proteins/metabolism*
PubMed: 17085079 Full text @ Gene Expr. Patterns
The Notch signaling pathway plays important roles in the regulation of diverse developmental processes. Although many Notch-signal target genes with different specificities have been identified, their regulation and functions are not fully understood. Here, we conducted a microarray screen to search for novel downstream target genes of the Notch pathway in zebrafish. From the screen, we isolated nort (Notch-regulated transcript) as a transcript whose expression was reduced by the inhibition of Notch signaling. The expression level of nort increased when Notch signaling was activated. nort was expressed in hypoblast cells and the developing nervous system. We found its expression pattern to be similar to that of her4, but it showed some differences, at least in the anterior and posterior neural plate at the 3-somite stage. The nort transcript did not contain any long open-reading frame (ORF) of more than 300nt, and its ORF-encoded sequence showed no significant homology with the proteins in databases. However, nort has one SPS (suppressor of hairless paired binding site) in its 5'-flanking region. These data suggest that nort is a putative noncoding RNA regulated by Notch signaling.