PUBLICATION
Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22
- Authors
- Montaville, P., Dai, Y., Cheung, C.Y., Giller, K., Becker, S., Michalak, M., Webb, S.E., Miller, A.L., and Krebs, J.
- ID
- ZDB-PUB-061020-48
- Date
- 2006
- Source
- Biochimica et biophysica acta. Molecular cell research 1763(11): 1335-1343 (Journal)
- Registered Authors
- Miller, Andrew L., Webb, Sarah E.
- Keywords
- ALG-2, RBM22; Ca2+-binding protein; RNA-binding protein; Zebrafish development; Confocal microscopy
- MeSH Terms
-
- Active Transport, Cell Nucleus
- Amino Acid Sequence
- Animals
- Apoptosis Regulatory Proteins/analysis
- Apoptosis Regulatory Proteins/genetics
- Apoptosis Regulatory Proteins/metabolism*
- Calcium Signaling
- Calcium-Binding Proteins/analysis
- Calcium-Binding Proteins/genetics
- Calcium-Binding Proteins/metabolism*
- Cell Nucleus/chemistry
- Cell Nucleus/metabolism*
- Humans
- Mice
- Molecular Sequence Data
- NIH 3T3 Cells
- RNA-Binding Proteins/analysis
- RNA-Binding Proteins/genetics
- RNA-Binding Proteins/metabolism*
- Transfection
- Two-Hybrid System Techniques
- Zebrafish/embryology
- Zebrafish/metabolism
- PubMed
- 17045351 Full text @ BBA Molecular Cell Research
Citation
Montaville, P., Dai, Y., Cheung, C.Y., Giller, K., Becker, S., Michalak, M., Webb, S.E., Miller, A.L., and Krebs, J. (2006) Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. Biochimica et biophysica acta. Molecular cell research. 1763(11):1335-1343.
Abstract
By yeast two-hybrid screening using the calcium-binding protein ALG-2 as bait a new target of ALG-2 was identified, the RNA-binding protein RBM22. In order to confirm these interactions in vivo we prepared fluorescent constructs by using the monomeric red fluorescent protein to label ALG-2 and the enhanced green fluorescent protein to label RBM22. Confocal microscopy of NIH 3T3 cells transfected with either ALG-2 or RBM22 expression constructs encoding fluorescent fusion proteins alone revealed that the majority of ALG-2 was localized in the cytoplasm whereas RBM22 was located in the nucleus. When cells were co-transfected with expression vectors encoding both fusion proteins ALG-2 was found in the nucleus indicating that RBM22 which can shuttle between the cytoplasm and the nucleus may play a role in nuclear translocation of ALG-2. Using zebrafish as a model mRNA homologues of ALG-2 and RBM22 were microinjected into the blastodisc-yolk margin of zebrafish embryos at the 1-cell stage followed by monitoring the fusion proteins during development of the zebrafish. Hereby, we observed that ALG-2 alone evenly distributed within the cell, whereas in the presence of RBM22 the two proteins co-localized within the nucleus. More than 95% of the two proteins co-localized within the same area in the nucleus suggesting a functional interaction between the Ca(2+)-signaling protein ALG-2 and the RNA-binding protein RBM22.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping