ZFIN ID: ZDB-PUB-060719-2
FGF signaling mediates regeneration of the differentiating cerebellum through repatterning of the anterior hindbrain and reinitiation of neuronal migration
Koster, R.W., and Fraser, S.E.
Date: 2006
Source: The Journal of neuroscience : the official journal of the Society for Neuroscience   26(27): 7293-7304 (Journal)
Registered Authors: Fraser, Scott E., Köster, Reinhard W.
Keywords: zebrafish, cerebellum, regeneration, plasticity, neuronal migration, bioimaging
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Cell Differentiation/physiology
  • Cell Movement/physiology*
  • Cerebellum/cytology
  • Cerebellum/embryology*
  • Cerebellum/physiology*
  • Denervation
  • Embryo, Nonmammalian/physiology
  • Fibroblast Growth Factor 8/genetics
  • Fibroblast Growth Factor 8/metabolism*
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins/genetics
  • Nerve Regeneration/physiology*
  • Neuronal Plasticity/physiology
  • Neurons/cytology
  • Neurons/physiology
  • Phenotype
  • Rhombencephalon/cytology
  • Rhombencephalon/embryology*
  • Rhombencephalon/physiology*
  • Signal Transduction/physiology
  • Zebrafish
PubMed: 16822987 Full text @ J. Neurosci.
To address the regenerative capability of the differentiating hindbrain, we ablated the cerebellum in wild-type and transgenic zebrafish embryos. These larvae showed no obvious locomotive malfunction several days after the ablation. Expression analysis and in vivo time-lapse recording in GFP (green fluorescent protein)-transgenic embryos indicate that cerebellar neuronal cells can regenerate from the remaining anterior hindbrain. The onset of regeneration is accompanied by repatterning within the anterior hindbrain. Inhibition of FGF signaling immediately after cerebellar ablation results in the lack of regenerating cerebellar neuronal cells and the absence of cerebellar structures several days later. Moreover, impaired FGF signaling inhibits the repatterning of the anterior hindbrain and the reexpression of rhombic lip marker genes soon after cerebellar ablation. This demonstrates that the hindbrain is highly plastic in recapitulating early embryonic differentiation mechanisms during regeneration. Moreover, the regenerating system offers a means to uncouple cerebellar differentiation from complex morphogenetic tissue rearrangements.