PUBLICATION
Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3
- Authors
- Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhauser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., and Bally-Cuif, L.
- ID
- ZDB-PUB-060710-9
- Date
- 2006
- Source
- Development (Cambridge, England) 133(15): 2925-2935 (Journal)
- Registered Authors
- Bally-Cuif, Laure, Becker, Thomas S., Geling, Andrea, Houart, Corinne, Kikuta, Hiroshi, Laplante, Mary, Ninkovic, Jovica, Stigloher, Christian, Topp, Stefanie
- Keywords
- Zebrafish, Telencephalon, Eye field, Forebrain, Rx3
- MeSH Terms
-
- Amino Acid Sequence
- Base Sequence
- Eye/embryology*
- Gene Deletion
- Prosencephalon/embryology*
- DNA Primers
- Gastrula/physiology
- Telencephalon/embryology*
- Homeostasis
- Visual Fields/physiology*
- Ethylnitrosourea/pharmacology
- Zebrafish/embryology*
- Animals
- Polymerase Chain Reaction
- Homeodomain Proteins/genetics
- Homeodomain Proteins/physiology*
- PubMed
- 16818451 Full text @ Development
- CTD
- 16818451
Citation
Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhauser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., and Bally-Cuif, L. (2006) Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3. Development (Cambridge, England). 133(15):2925-2935.
Abstract
Anteroposterior patterning of the vertebrate forebrain during gastrulation involves graded Wnt signaling, which segregates anterior fields (telencephalon and eye) from the diencephalon. How the telencephalic and retinal primordia are subsequently subdivided remains largely unknown. We demonstrate that at late gastrulation the Paired-like homeodomain transcription factor Rx3 biases cell specification choices towards the retinal fate within a population of bipotential precursors of the anterior forebrain: direct cell tracing demonstrates that retinal precursors acquire a telencephalic fate in embryos homozygous for the rx3-null allele ckh(ne2611), characterized by an enlarged telencephalon and a lack of eyes. Chimera analyses further indicate that this function of Rx3 is cell autonomous. Transfating of the eye field in the absence of Rx3 function correlates with a substantial posterior expansion of expression of the Wnt antagonist Tlc and the winged-helix transcription factor Foxg1. These results suggest that the process segregating the telencephalic and eye fields is isolated from diencephalic patterning, and is mediated by Rx3.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping