PUBLICATION

Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3

Authors
Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhauser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., and Bally-Cuif, L.
ID
ZDB-PUB-060710-9
Date
2006
Source
Development (Cambridge, England)   133(15): 2925-2935 (Journal)
Registered Authors
Bally-Cuif, Laure, Becker, Thomas S., Geling, Andrea, Houart, Corinne, Kikuta, Hiroshi, Laplante, Mary, Ninkovic, Jovica, Stigloher, Christian, Topp, Stefanie
Keywords
Zebrafish, Telencephalon, Eye field, Forebrain, Rx3
MeSH Terms
  • Animals
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/physiology*
  • Gastrula/physiology
  • Ethylnitrosourea/pharmacology
  • Prosencephalon/embryology*
  • DNA Primers
  • Amino Acid Sequence
  • Homeostasis
  • Base Sequence
  • Polymerase Chain Reaction
  • Gene Deletion
  • Visual Fields/physiology*
  • Zebrafish/embryology*
  • Telencephalon/embryology*
  • Eye/embryology*
(all 16)
PubMed
16818451 Full text @ Development
CTD
16818451
Abstract
Anteroposterior patterning of the vertebrate forebrain during gastrulation involves graded Wnt signaling, which segregates anterior fields (telencephalon and eye) from the diencephalon. How the telencephalic and retinal primordia are subsequently subdivided remains largely unknown. We demonstrate that at late gastrulation the Paired-like homeodomain transcription factor Rx3 biases cell specification choices towards the retinal fate within a population of bipotential precursors of the anterior forebrain: direct cell tracing demonstrates that retinal precursors acquire a telencephalic fate in embryos homozygous for the rx3-null allele ckh(ne2611), characterized by an enlarged telencephalon and a lack of eyes. Chimera analyses further indicate that this function of Rx3 is cell autonomous. Transfating of the eye field in the absence of Rx3 function correlates with a substantial posterior expansion of expression of the Wnt antagonist Tlc and the winged-helix transcription factor Foxg1. These results suggest that the process segregating the telencephalic and eye fields is isolated from diencephalic patterning, and is mediated by Rx3.
Genes / Markers
Figures
Figure Gallery (10 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
hu499
    		Point Mutation
    ne2611
      		Point Mutation
      s399
        		Point Mutation
        w29
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          rx3hu499/hu499
          rx3ne2611/ne2611
          rx3s399/s399
          rx3w29/w29 (AB)
          WT
          1 - 5 of 5
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          Antibodies
          No data available
          Orthology
          No data available
          Engineered Foreign Genes
          No data available
          Mapping
          No data available
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          Citation
          Stigloher, C., Ninkovic, J., Laplante, M., Geling, A., Tannhauser, B., Topp, S., Kikuta, H., Becker, T.S., Houart, C., and Bally-Cuif, L. (2006) Segregation of telencephalic and eye-field identities inside the zebrafish forebrain territory is controlled by Rx3. Development (Cambridge, England). 133(15):2925-2935.