PUBLICATION
Structural and functional characterization of the zebrafish lamin B receptor
- Authors
- Schild-Prufert, K., Giegerich, M., Schafer, M., Winkler, C., and Krohne, G.
- ID
- ZDB-PUB-060616-40
- Date
- 2006
- Source
- European journal of cell biology 85(8): 813-824 (Journal)
- Registered Authors
- Schafer, Matthias, Winkler, Christoph
- Keywords
- Zebrafish, Lamin B receptor, LBR gene, Morpholino oligonucleotide, Nuclear envelope
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- Electrophoresis, Polyacrylamide Gel
- Embryo, Nonmammalian/cytology
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Exons/genetics
- Fetal Proteins
- Gene Expression Regulation, Developmental
- Gene Silencing
- Goosecoid Protein/genetics
- Goosecoid Protein/metabolism
- Humans
- Immunoblotting
- Introns/genetics
- Molecular Sequence Data
- Mutation
- Receptors, Cytoplasmic and Nuclear/genetics*
- Receptors, Cytoplasmic and Nuclear/metabolism
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- T-Box Domain Proteins/genetics
- T-Box Domain Proteins/metabolism
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 16759737 Full text @ Eur. J. Cell Biol.
Citation
Schild-Prufert, K., Giegerich, M., Schafer, M., Winkler, C., and Krohne, G. (2006) Structural and functional characterization of the zebrafish lamin B receptor. European journal of cell biology. 85(8):813-824.
Abstract
The lamin B receptor (LBR) is an integral membrane protein of the inner nuclear membrane that is interacting with B-type lamins, chromatin and DNA. The complete loss of the protein in mouse mutants causes a reduced viability of embryos, and viable animals develop abnormalities of the skeleton. Here, we present the molecular characterization of the zebrafish LBR (zLBR) gene and the functional analysis of LBR during zebrafish embryogenesis. We found that the coding region of the LBR mRNA of zebrafish as well as of mammals is contained in 13 exons. At the protein level, human and zebrafish LBR exhibit a high sequence identity (57% and higher) in 8 of the 13 exons. Knockdown of zLBR by microinjection of 0.5-1.0mM morpholino antisense oligonucleotides (MO) into 1- to 2-cell stage embryos reduced the amount of endogenous zLBR protein to approximately 10-20%. The viability of MO-injected embryos within 24h was reduced to 70-77%. Surviving 1-day-old embryos exhibited morphological alterations including reduced growth of head structures, retardation of tail growth and a bent backbone and tail. Expression analysis of the transcription factors no tail (ntl) and goosecoid (gsc) by in situ hybridization suggests that these malformations are caused by altered cell migration during gastrulation. Our data indicate that the LBR of zebrafish and mammals are both required for correct development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping