PUBLICATION
MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model
- Authors
- van der Sar, A.M., Stockhammer, O.W., van der Laan, C., Spaink, H.P., Bitter, W., and Meijer, A.H.
- ID
- ZDB-PUB-060323-32
- Date
- 2006
- Source
- Infection and Immunity 74(4): 2436-2441 (Journal)
- Registered Authors
- Meijer, Annemarie H., Spaink, Herman P.
- Keywords
- none
- MeSH Terms
-
- Adaptor Proteins, Signal Transducing/genetics
- Adaptor Proteins, Signal Transducing/physiology*
- Toll-Like Receptors/genetics
- Toll-Like Receptors/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Immunity, Innate*/genetics
- Salmonella typhimurium/genetics
- Salmonella typhimurium/immunology
- Myeloid Differentiation Factor 88
- Molecular Sequence Data
- Disease Models, Animal
- Animals
- Bacterial Outer Membrane Proteins/biosynthesis*
- Bacterial Outer Membrane Proteins/genetics
- Bacterial Outer Membrane Proteins/metabolism
- Signal Transduction/genetics
- Signal Transduction/immunology*
- Salmonella Infections/genetics
- Salmonella Infections/immunology
- Oligonucleotides, Antisense/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/immunology*
- PubMed
- 16552074 Full text @ Infect. Immun.
Citation
van der Sar, A.M., Stockhammer, O.W., van der Laan, C., Spaink, H.P., Bitter, W., and Meijer, A.H. (2006) MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model. Infection and Immunity. 74(4):2436-2441.
Abstract
Innate immunity signaling mechanisms during vertebrate embryogenesis are largely unknown. To study Toll-like receptor (TLR) signaling function in the zebrafish embryo model, we designed an experimental setup for antisense morpholino knockdown under conditions of bacterial infection. Clearance of Salmonella enterica serovar Typhimurium Ra bacteria was significantly impaired after knockdown of myeloid differentiation factor 88 (MyD88), a common adaptor protein in TLR and interleukin-1 receptor signaling. Thereby, we demonstrate for the first time that the innate immune response of the developing embryo involves MyD88-dependent signaling, which further establishes the zebrafish embryo as a model for the study of vertebrate innate immunity.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping