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ZFIN ID: ZDB-PUB-060323-32
MyD88 Innate Immune Function in a Zebrafish Embryo Infection Model
van der Sar, A.M., Stockhammer, O.W., van der Laan, C., Spaink, H.P., Bitter, W., and Meijer, A.H.
Date: 2006
Source: Infection and Immunity   74(4): 2436-2441 (Journal)
Registered Authors: Meijer, Annemarie H., Spaink, Herman P.
Keywords: none
MeSH Terms:
  • Adaptor Proteins, Signal Transducing/genetics
  • Adaptor Proteins, Signal Transducing/physiology*
  • Animals
  • Bacterial Outer Membrane Proteins/biosynthesis*
  • Bacterial Outer Membrane Proteins/genetics
  • Bacterial Outer Membrane Proteins/metabolism
  • Disease Models, Animal
  • Immunity, Innate*/genetics
  • Molecular Sequence Data
  • Myeloid Differentiation Factor 88
  • Oligonucleotides, Antisense/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Salmonella Infections/genetics
  • Salmonella Infections/immunology
  • Salmonella typhimurium/genetics
  • Salmonella typhimurium/immunology
  • Signal Transduction/genetics
  • Signal Transduction/immunology*
  • Toll-Like Receptors/genetics
  • Toll-Like Receptors/physiology
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/immunology*
PubMed: 16552074 Full text @ Infect. Immun.
Innate immunity signaling mechanisms during vertebrate embryogenesis are largely unknown. To study Toll-like receptor (TLR) signaling function in the zebrafish embryo model, we designed an experimental setup for antisense morpholino knockdown under conditions of bacterial infection. Clearance of Salmonella enterica serovar Typhimurium Ra bacteria was significantly impaired after knockdown of myeloid differentiation factor 88 (MyD88), a common adaptor protein in TLR and interleukin-1 receptor signaling. Thereby, we demonstrate for the first time that the innate immune response of the developing embryo involves MyD88-dependent signaling, which further establishes the zebrafish embryo as a model for the study of vertebrate innate immunity.