PUBLICATION

Hedgehog signalling controls zebrafish neural keel morphogenesis via its level-dependent effects on neurogenesis

Authors
Takamiya, M., and Campos-Ortega, J.A.
ID
ZDB-PUB-060313-5
Date
2006
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   235(4): 978-997 (Journal)
Registered Authors
Campos-Ortega, Jose, Takamiya, Masanari
Keywords
zebrafish, polarity, Hh, neurulation, morphogenesis
MeSH Terms
  • Cell Polarity
  • Embryo, Nonmammalian
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Hedgehog Proteins
  • Trans-Activators/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Signal Transduction*
  • Morphogenesis*
  • Microinjections
  • Nervous System/cytology
  • Nervous System/embryology*
  • Animals, Genetically Modified
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Animals
  • In Situ Hybridization
(all 19)
PubMed
16502420 Full text @ Dev. Dyn.
Abstract
We investigated the role of hedgehog (Hh) signalling on zebrafish neurulation, focusing on the intimate relationship between neurogenesis and morphogenesis during the neural keel stage. Through the analyses of Hh loss- and gain-of-function phenotypes, we found that Hh signalling controls the neural keel morphogenesis. To investigate underlying mechanisms, we examined cellular elongation polarity in the neural keel of Hh loss- and gain-of-function phenotypes and compared this with the deficient phenotype of a planar cell polarity (PCP) molecule, Trilobite/Strabismus. We found that Hh signalling controls cell elongation polarity of the neuroepithelium at least in part by means of PCP pathway; however, its effects are not strong enough per se to affect keel morphogenesis; instead Hh signalling mainly controls keel morphogenesis by means of affecting both medial and lateral neurogenesis. We devised a method for precise evaluation of neurogenesis in loss- and gain-of-Hh phenotypes that compensates for its delay caused by disturbed morphogenesis. We present a model that Hh signalling exerts level-dependent and binary-opposite effects on medial neurogenesis, whose modification to explain lateral neurogenesis reveals regional differences of underlying mechanisms between the two proneural domains. Such differences seem to be created in part by regional effector signalling; the effects of high Hh-signalling on medial neurogenesis can be reversed in accordance to medial Tri/Stbm level, in a polarity independent manner.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    kca4TgTransgenic Insertion
      kca7TgTransgenic Insertion
        1 - 3 of 3
        Show
        Human Disease / Model
        No data available
        Sequence Targeting Reagents
        Target Reagent Reagent Type
        vangl2MO1-vangl2MRPHLNO
        1 - 1 of 1
        Show
        Fish
        Fish
        kca4Tg
        kca7Tg
        smob641/b641 (AB)
        WT
        WT + MO1-vangl2
        1 - 5 of 5
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        Antibodies
        No data available
        Orthology
        No data available
        Engineered Foreign Genes
        No data available
        Mapping
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        Citation
        Takamiya, M., and Campos-Ortega, J.A. (2006) Hedgehog signalling controls zebrafish neural keel morphogenesis via its level-dependent effects on neurogenesis. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):978-997.