PUBLICATION
Polycystin-1, STAT6, and P100 Function in a Pathway that Transduces Ciliary Mechanosensation and Is Activated in Polycystic Kidney Disease
- Authors
- Low, S.H., Vasanth, S., Larson, C.H., Mukherjee, S., Sharma, N., Kinter, M.T., Kane, M.E., Obara, T., and Weimbs, T.
- ID
- ZDB-PUB-060124-3
- Date
- 2006
- Source
- Developmental Cell 10(1): 57-69 (Journal)
- Registered Authors
- Obara, Tomoko
- Keywords
- none
- MeSH Terms
-
- Cell Line
- Polycystic Kidney, Autosomal Dominant/metabolism*
- Polycystic Kidney, Autosomal Dominant/pathology
- Nuclear Proteins/metabolism*
- Protein Binding
- Humans
- Epithelium/drug effects
- Epithelium/metabolism
- Fluorescent Antibody Technique/methods
- Trans-Activators/physiology
- Gene Expression/physiology
- Cilia/drug effects
- Cilia/metabolism*
- Proteins/physiology*
- Enzyme Activation/physiology
- Immunoprecipitation/methods
- Dose-Response Relationship, Drug
- Mechanotransduction, Cellular/physiology*
- Molecular Biology/methods
- Embryo, Nonmammalian
- Luciferases/metabolism
- Transfection/methods
- Translocation, Genetic
- Animals
- TRPP Cation Channels
- Interleukin-4/pharmacology
- STAT6 Transcription Factor/metabolism*
- Gene Expression Regulation/drug effects
- Gene Expression Regulation/physiology
- Models, Biological
- Protein Structure, Tertiary
- Amino Acid Sequence
- Zebrafish
- Mutagenesis/physiology
- Blotting, Northern/methods
- Kidney/metabolism
- Kidney/pathology
- Kidney/ultrastructure
- Blotting, Western/methods
- Embryo, Mammalian
- PubMed
- 16399078 Full text @ Dev. Cell
Citation
Low, S.H., Vasanth, S., Larson, C.H., Mukherjee, S., Sharma, N., Kinter, M.T., Kane, M.E., Obara, T., and Weimbs, T. (2006) Polycystin-1, STAT6, and P100 Function in a Pathway that Transduces Ciliary Mechanosensation and Is Activated in Polycystic Kidney Disease. Developmental Cell. 10(1):57-69.
Abstract
Primary cilia are implicated in the pathogenesis of autosomal-dominant polycystic kidney disease (ADPKD), which results from defects in polycystin-1 (PC1), but the function of PC1 remains poorly understood. Here, we show that PC1 undergoes proteolytic cleavage that results in nuclear translocation of its cytoplasmic tail. The PC1 tail interacts with the transcription factor STAT6 and the coactivator P100, and it stimulates STAT6-dependent gene expression. Under normal conditions, STAT6 localizes to primary cilia of renal epithelial cells. Cessation of apical fluid flow results in nuclear translocation of STAT6. Cyst-lining cells in ADPKD exhibit elevated levels of nuclear STAT6, P100, and the PC1 tail. Exogenous expression of the human PC1 tail results in renal cyst formation in zebrafish embryos. These results identify a novel mechanism of cilia function in the transduction of a mechanical signal to changes of gene expression involving PC1 and show that this pathway is inappropriately activated in ADPKD.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping