| ZFIN ID: ZDB-PUB-060124-11 |
High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
Burns, C.G., Milan, D.J., Grande, E.J., Rottbauer, W., MacRae, C.A., and Fishman, M.C.
| Date: | 2005 |
|---|---|
| Source: | Nature Chemical Biology 1(5): 263-264 (Journal) |
| Registered Authors: | Burns, Geoff, Fishman, Mark C., MacRae, Calum A., Milan, David J., Rottbauer, Wolfgang |
| Keywords: | none |
| MeSH Terms: |
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| PubMed: | 16408054 Full text @ Nat. Chem. Biol. |
Citation
Burns, C.G., Milan, D.J., Grande, E.J., Rottbauer, W., MacRae, C.A., and Fishman, M.C. (2005) High-throughput assay for small molecules that modulate zebrafish embryonic heart rate. Nature Chemical Biology. 1(5):263-264.
ABSTRACT
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
ADDITIONAL INFORMATION
- Genes / Markers (1)
- Mutations and Transgenics (1)
- Fish (1)