PUBLICATION
High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
- Authors
- Burns, C.G., Milan, D.J., Grande, E.J., Rottbauer, W., MacRae, C.A., and Fishman, M.C.
- ID
- ZDB-PUB-060124-11
- Date
- 2005
- Source
- Nature Chemical Biology 1(5): 263-264 (Journal)
- Registered Authors
- Burns, Geoff, Fishman, Mark C., MacRae, Calum A., Milan, David J., Rottbauer, Wolfgang
- Keywords
- none
- MeSH Terms
-
- Drug Evaluation, Preclinical/methods
- Cardiovascular Agents/toxicity*
- Animals
- Feasibility Studies
- Myocardium/metabolism
- Biological Assay/methods*
- Zebrafish
- Embryo, Nonmammalian/physiology
- Heart/drug effects*
- Heart/embryology
- Heart/physiology*
- Time Factors
- Dose-Response Relationship, Drug
- Heart Rate/drug effects*
- Heart Rate/physiology
- Green Fluorescent Proteins/analysis*
- PubMed
- 16408054 Full text @ Nat. Chem. Biol.
Citation
Burns, C.G., Milan, D.J., Grande, E.J., Rottbauer, W., MacRae, C.A., and Fishman, M.C. (2005) High-throughput assay for small molecules that modulate zebrafish embryonic heart rate. Nature Chemical Biology. 1(5):263-264.
Abstract
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping