ZFIN ID: ZDB-PUB-060124-11
High-throughput assay for small molecules that modulate zebrafish embryonic heart rate
Burns, C.G., Milan, D.J., Grande, E.J., Rottbauer, W., MacRae, C.A., and Fishman, M.C.
Date: 2005
Source: Nature Chemical Biology   1(5): 263-264 (Journal)
Registered Authors: Burns, Geoff, Fishman, Mark C., MacRae, Calum A., Milan, David J., Rottbauer, Wolfgang
Keywords: none
MeSH Terms:
  • Animals
  • Biological Assay/methods*
  • Cardiovascular Agents/toxicity*
  • Dose-Response Relationship, Drug
  • Drug Evaluation, Preclinical/methods
  • Embryo, Nonmammalian/physiology
  • Feasibility Studies
  • Green Fluorescent Proteins/analysis*
  • Heart/drug effects*
  • Heart/embryology
  • Heart/physiology*
  • Heart Rate/drug effects*
  • Heart Rate/physiology
  • Myocardium/metabolism
  • Time Factors
  • Zebrafish
PubMed: 16408054 Full text @ Nat. Chem. Biol.
ABSTRACT
To increase the facility and throughput of scoring phenotypic traits in embryonic zebrafish, we developed an automated micro-well assay for heart rate using automated fluorescence microscopy of transgenic embryos expressing green fluorescent protein in myocardium. The assay measures heart rates efficiently and accurately over a large linear dynamic range, and it rapidly characterizes dose dependence and kinetics of small molecule-induced changes in heart rate. This is the first high-throughput micro-well assay for organ function in an intact vertebrate.
ADDITIONAL INFORMATION