PUBLICATION

The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney

Authors
Hong, S.K., Haldin, C.E., Lawson, N.D., Weinstein, B.M., Dawid, I.B., and Hukriede, N.A.
ID
ZDB-PUB-051214-26
Date
2005
Source
Proceedings of the National Academy of Sciences of the United States of America   102(51): 18473-18478 (Journal)
Registered Authors
Dawid, Igor B., Haldin, Caroline, Hong, Sung-Kook, Hukriede, Neil, Lawson, Nathan, Weinstein, Brant M.
Keywords
branchial arches, Danio rerio, Mediator, morphogenesis, TRAP230
MeSH Terms
  • Kidney/embryology*
  • Kidney/metabolism
  • Animals
  • Eye Proteins/genetics
  • Eye Proteins/metabolism*
  • Neural Crest/embryology*
  • Neural Crest/metabolism
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Phenotype
  • Base Sequence
  • Molecular Sequence Data
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Drosophila Proteins/genetics
  • Drosophila Proteins/metabolism*
  • Cloning, Molecular
  • Brain/embryology*
  • Brain/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
  • Mediator Complex
  • Mutation/genetics
  • Gene Expression Regulation, Developmental
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
(all 27)
PubMed
16344459 Full text @ Proc. Natl. Acad. Sci. USA
CTD
16344459
Abstract
Mutation of the gene encoding the Mediator component thyroid hormone receptor-associated protein (TRAP)230/MED12 affects the development of multiple systems in zebrafish embryogenesis. We isolated two ethylnitrosourea-induced alleles in the gene encoding this protein and named the locus kohtalo (kto) after the homologous locus in Drosophila. Homozygous kto mutant zebrafish embryos show defects in brain, neural crest, and kidney development and die at approximately 6 days postfertilization. In the affected tissues, differentiation is initiated and many cell type-specific genes are expressed, but there is a failure of morphogenesis and failure to complete differentiation. These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly.
Genes / Markers
Figures
Figure Gallery (7 images)
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Expression
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay Figure
med12y81/y81standard conditionsPrim-5ISH
med12y82/y82standard conditionsPrim-5ISH
WTstandard conditionsPrim-5ISH
WTstandard conditionsPrim-5ISH
WTstandard conditionsPrim-5ISH
WTstandard conditionsPrim-5ISH
med12y81/y81standard conditionsPrim-25ISH
med12y81/y81standard conditionsPrim-25Not DetectedISH
med12y81/y81standard conditionsPrim-25ISH
med12y82/y82standard conditionsPrim-25ISH
1 - 10 of 77
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Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
y81
    		Point Mutation
    y82
      		Point Mutation
      1 - 2 of 2
      Show
      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      med12MO1-med12MRPHLNO
      1 - 1 of 1
      Show
      Fish
      Fish
      med12y81/y81
      med12y82/y82
      WT
      WT + MO1-med12
      1 - 4 of 4
      Show
      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      Entity Type Entity Symbol Location
      GENEmed12Chr: 14 Details
      GENEslit3Chr: 14 Details
      SSLPz7495Chr: 14 Details
      SSLPz22094Chr: 14 Details
      SSLPz22128Chr: 14 Details
      SSLPz34510Chr: 14 Details
      1 - 6 of 6
      Show
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      Citation
      Hong, S.K., Haldin, C.E., Lawson, N.D., Weinstein, B.M., Dawid, I.B., and Hukriede, N.A. (2005) The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney. Proceedings of the National Academy of Sciences of the United States of America. 102(51):18473-18478.