ZFIN ID: ZDB-PUB-051214-26
The zebrafish kohtalo/trap230 gene is required for the development of the brain, neural crest, and pronephric kidney
Hong, S.K., Haldin, C.E., Lawson, N.D., Weinstein, B.M., Dawid, I.B., and Hukriede, N.A.
Date: 2005
Source: Proceedings of the National Academy of Sciences of the United States of America   102(51): 18473-18478 (Journal)
Registered Authors: Dawid, Igor B., Haldin, Caroline, Hong, Sung-Kook, Hukriede, Neil, Lawson, Nathan, Weinstein, Brant M.
Keywords: branchial arches, Danio rerio, Mediator, morphogenesis, TRAP230
MeSH Terms:
  • Animals
  • Base Sequence
  • Brain/embryology*
  • Brain/metabolism
  • Cloning, Molecular
  • Drosophila Proteins/genetics
  • Drosophila Proteins/metabolism*
  • Embryo, Nonmammalian/embryology
  • Embryo, Nonmammalian/metabolism
  • Eye Proteins/genetics
  • Eye Proteins/metabolism*
  • Gene Expression Regulation, Developmental
  • Kidney/embryology*
  • Kidney/metabolism
  • Mediator Complex
  • Molecular Sequence Data
  • Mutation/genetics
  • Neural Crest/embryology*
  • Neural Crest/metabolism
  • Phenotype
  • Transcription Factors/genetics
  • Transcription Factors/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 16344459 Full text @ Proc. Natl. Acad. Sci. USA
Mutation of the gene encoding the Mediator component thyroid hormone receptor-associated protein (TRAP)230/MED12 affects the development of multiple systems in zebrafish embryogenesis. We isolated two ethylnitrosourea-induced alleles in the gene encoding this protein and named the locus kohtalo (kto) after the homologous locus in Drosophila. Homozygous kto mutant zebrafish embryos show defects in brain, neural crest, and kidney development and die at approximately 6 days postfertilization. In the affected tissues, differentiation is initiated and many cell type-specific genes are expressed, but there is a failure of morphogenesis and failure to complete differentiation. These results suggest that critical targets of TRAP230 function may include proteins important for cell mobility, cell sorting, and tissue assembly.