PUBLICATION
Wnt5 signaling in vertebrate pancreas development
- Authors
- Kim, H.J., Schleiffarth, J.R., Jessurun, J., Sumanas, S., Petryk, A., Lin, S., and Ekker, S.C.
- ID
- ZDB-PUB-051101-8
- Date
- 2005
- Source
- BMC Biology 3: 23 (Journal)
- Registered Authors
- Ekker, Stephen C., Lin, Shuo, Sumanas, Saulius
- Keywords
- none
- MeSH Terms
-
- Animals
- Base Sequence
- Cell Movement
- Embryo, Nonmammalian/physiology
- Frizzled Receptors
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Insulin/genetics
- Islets of Langerhans/embryology
- Mice
- Mice, Knockout
- Morphogenesis
- Pancreas/embryology*
- Polymerase Chain Reaction
- Proto-Oncogene Proteins/genetics*
- Proto-Oncogene Proteins/physiology*
- Receptors, G-Protein-Coupled/genetics*
- Recombinant Fusion Proteins/metabolism
- Signal Transduction
- Transcription, Genetic
- Transfection
- Vertebrates/embryology*
- Vertebrates/growth & development
- Wnt Proteins/genetics*
- Wnt Proteins/physiology
- Xenopus
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/physiology*
- PubMed
- 16246260 Full text @ BMC Biol.
Citation
Kim, H.J., Schleiffarth, J.R., Jessurun, J., Sumanas, S., Petryk, A., Lin, S., and Ekker, S.C. (2005) Wnt5 signaling in vertebrate pancreas development. BMC Biology. 3:23.
Abstract
BACKGROUND: Signaling by the Wnt family of secreted glycoproteins through their receptors, the frizzled (Fz) family of seven-pass transmembrane proteins, is critical for numerous cell fate and tissue polarity decisions during development. RESULTS: We report a novel role of Wnt signaling in organogenesis using the formation of the islet during pancreatic development as a model tissue. We used the advantages of the zebrafish to visualize and document this process in living embryos and demonstrated that insulin-positive cells actively migrate to form an islet. We used morpholinos (MOs), sequence-specific translational inhibitors, and time-lapse imaging analysis to show that the Wnt-5 ligand and the Fz-2 receptor are required for proper insulin-cell migration in zebrafish. Histological analyses of islets in Wnt5a-/- mouse embryos showed that Wnt5a signaling is also critical for murine pancreatic insulin-cell migration. CONCLUSIONS: Our results implicate a conserved role of a Wnt5/Fz2 signaling pathway in islet formation during pancreatic development. This study opens the door for further investigation into a role of Wnt signaling in vertebrate organ development and disease.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping