PUBLICATION
Patterns of virulence gene expression differ between biofilm and tissue communities of Streptococcus pyogenes
- Authors
- Cho, K.H., and Caparon, M.G.
- ID
- ZDB-PUB-050907-9
- Date
- 2005
- Source
- Molecular Microbiology 57(6): 1545-1556 (Journal)
- Registered Authors
- Caparon, Michael
- Keywords
- none
- MeSH Terms
-
- Animals
- Bacterial Proteins/genetics
- Bacterial Proteins/metabolism*
- Biofilms/growth & development*
- Disease Models, Animal
- Ecosystem
- Gene Expression Regulation, Bacterial*
- Humans
- Mutation
- Oligonucleotide Array Sequence Analysis
- Soft Tissue Infections/microbiology
- Soft Tissue Infections/physiopathology*
- Streptococcal Infections/microbiology
- Streptococcal Infections/physiopathology*
- Streptococcus pyogenes/genetics
- Streptococcus pyogenes/growth & development
- Streptococcus pyogenes/pathogenicity*
- Transcription, Genetic
- Virulence
- Zebrafish
- PubMed
- 16135223 Full text @ Mol. Microbiol.
Citation
Cho, K.H., and Caparon, M.G. (2005) Patterns of virulence gene expression differ between biofilm and tissue communities of Streptococcus pyogenes. Molecular Microbiology. 57(6):1545-1556.
Abstract
The ability of Streptococcus pyogenes to form biofilm-like bacterial communities during infection of soft tissue has suggested that the capacity to produce biofilm may be important for pathogenesis. To examine this relationship, a panel of mutants was evaluated for their ability to form biofilm on abiotic surfaces in several assays. Several established virulence factors were crucial for biofilm formation, including the M protein, required for initial cell-surface interactions, and the hyaluronic acid capsule, required for subsequent maturation into a three-dimensional structure. Mutants lacking the transcription regulators Mga and CovR (CsrR) also failed to form biofilm. Comparison of transcriptional profiles revealed differential regulation of approximately 25% of the genome upon adaptation to biofilm. During infection of zebrafish, several virulence factors (notably cysteine protease and streptokinase) were regulated in a biofilm-like manner. However, the overall profile of virulence factor expression indicated that tissue communities have a pattern of gene expression different from biofilm. Taken together, these data show that while biofilm and tissue communities have many characteristics in common, that biofilm reproduces only a subset of the myriad cues used by tissue communities for regulation of virulence.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping