ZFIN ID: ZDB-PUB-050907-11
Vertebrate Smoothened functions at the primary cilium
Corbit, K.C., Aanstad, P., Singla, V., Norman, A.R., Stainier, D.Y., and Reiter, J.F.
Date: 2005
Source: Nature 437(7061): 1018-1021 (Journal)
Registered Authors: Aanstad, Pia, Norman, Andrew, Reiter, Jeremy, Stainier, Didier
Keywords: none
MeSH Terms:
  • Amino Acid Motifs
  • Amino Acid Sequence
  • Animals
  • Caenorhabditis elegans/chemistry
  • Cell Line
  • Cilia/drug effects
  • Cilia/metabolism*
  • Dogs
  • Drosophila Proteins/chemistry
  • Embryo, Mammalian/metabolism
  • Embryo, Nonmammalian
  • Genes, Reporter/genetics
  • Mice
  • Mutation/genetics
  • Protein Sorting Signals/genetics
  • Protein Sorting Signals/physiology
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Receptors, G-Protein-Coupled/antagonists & inhibitors
  • Receptors, G-Protein-Coupled/chemistry
  • Receptors, G-Protein-Coupled/genetics
  • Receptors, G-Protein-Coupled/metabolism*
  • Signal Transduction
  • Veratrum Alkaloids/pharmacology
  • Vertebrates/embryology
  • Vertebrates/genetics
  • Vertebrates/metabolism*
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
PubMed: 16136078 Full text @ Nature
FIGURES
ABSTRACT
The unanticipated involvement of several intraflagellar transport proteins in the mammalian Hedgehog (Hh) pathway has hinted at a functional connection between cilia and Hh signal transduction. Here we show that mammalian Smoothened (Smo), a seven-transmembrane protein essential for Hh signalling, is expressed on the primary cilium. This ciliary expression is regulated by Hh pathway activity; Sonic hedgehog or activating mutations in Smo promote ciliary localization, whereas the Smo antagonist cyclopamine inhibits ciliary localization. The translocation of Smo to primary cilia depends upon a conserved hydrophobic and basic residue sequence homologous to a domain previously shown to be required for the ciliary localization of seven-transmembrane proteins in Caenorhabditis elegans. Mutation of this domain not only prevents ciliary localization but also eliminates Smo activity both in cultured cells and in zebrafish embryos. Thus, Hh-dependent translocation to cilia is essential for Smo activity, suggesting that Smo acts at the primary cilium.
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